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Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs

The CRISPR/Cas9 genome editing tool has increased the efficiency of creating genetically modified pigs for use as biomedical or agricultural models. The objectives were to determine if DNA editing resulted in a delay in development to the blastocyst stage or in a skewing of the sex ratio. Six DNA te...

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Detalles Bibliográficos
Autores principales:	Whitworth, Kristin M., Benne, Joshua A., Spate, Lee D., Murphy, Stephanie L., Samuel, Melissa S., Murphy, Clifton N., Richt, Jürgen A., Walters, Eric, Prather, Randall S., Wells, Kevin D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer International Publishing 2016
Materias:	Original Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247313/ https://www.ncbi.nlm.nih.gov/pubmed/27744533 http://dx.doi.org/10.1007/s11248-016-9989-6

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