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Eliminating hydrolytic activity without affecting the transglycosylation of a GH1 β-glucosidase

Unveiling the determinants for transferase and hydrolase activity in glycoside hydrolases would allow using their vast diversity for creating novel transglycosylases, thereby unlocking an extensive toolbox for carbohydrate chemists. Three different amino acid substitutions at position 220 of a GH1 β...

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Detalles Bibliográficos
Autores principales: Lundemo, Pontus, Karlsson, Eva Nordberg, Adlercreutz, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247548/
https://www.ncbi.nlm.nih.gov/pubmed/27678115
http://dx.doi.org/10.1007/s00253-016-7833-9

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