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Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM

Monitoring of different signalling enzymes in a single assay using multiplex biosensing provides a multidimensional workspace to elucidate biological processes, signalling pathway crosstalk, and determine precise sequence of events at the single living cell level. In this study, we interrogate the c...

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Detalles Bibliográficos
Autores principales:	Demeautis, Claire, Sipieter, François, Roul, Julien, Chapuis, Catherine, Padilla-Parra, Sergi, Riquet, Franck B., Tramier, Marc
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247693/ https://www.ncbi.nlm.nih.gov/pubmed/28106114 http://dx.doi.org/10.1038/srep41026

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