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Modeling Stem Cell Myogenic Differentiation
The process of stem cell myogenesis (transformation into skeletal muscle cells) includes several stages characterized by the expression of certain combinations of myogenic factors. The first part of this process is accompanied by cell division, while the second part is mainly associated with direct...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247743/ https://www.ncbi.nlm.nih.gov/pubmed/28106095 http://dx.doi.org/10.1038/srep40639 |
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author | Deshpande, Rajiv S. Spector, Alexander A. |
author_facet | Deshpande, Rajiv S. Spector, Alexander A. |
author_sort | Deshpande, Rajiv S. |
collection | PubMed |
description | The process of stem cell myogenesis (transformation into skeletal muscle cells) includes several stages characterized by the expression of certain combinations of myogenic factors. The first part of this process is accompanied by cell division, while the second part is mainly associated with direct differentiation. The mechanical cues are known to enhance stem cell myogenesis, and the paper focuses on the stem cell differentiation under the condition of externally applied strain. The process of stem cell myogenic differentiation is interpreted as the interplay among transcription factors, targeted proteins and strain-generated signaling molecule, and it is described by a kinetic multi-stage model. The model parameters are optimally adjusted by using the available data from the experiment with adipose-derived stem cells subjected to the application of cyclic uniaxial strains of the magnitude of 10%. The modeling results predict the kinetics of the process of myogenic differentiation, including the number of cells in each stage of differentiation and the rates of differentiation from one stage to another for different strains from 4% to 16%. The developed model can help better understand the process of myogenic differentiation and the effects of mechanical cues on stem cell use in muscle therapies. |
format | Online Article Text |
id | pubmed-5247743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52477432017-01-23 Modeling Stem Cell Myogenic Differentiation Deshpande, Rajiv S. Spector, Alexander A. Sci Rep Article The process of stem cell myogenesis (transformation into skeletal muscle cells) includes several stages characterized by the expression of certain combinations of myogenic factors. The first part of this process is accompanied by cell division, while the second part is mainly associated with direct differentiation. The mechanical cues are known to enhance stem cell myogenesis, and the paper focuses on the stem cell differentiation under the condition of externally applied strain. The process of stem cell myogenic differentiation is interpreted as the interplay among transcription factors, targeted proteins and strain-generated signaling molecule, and it is described by a kinetic multi-stage model. The model parameters are optimally adjusted by using the available data from the experiment with adipose-derived stem cells subjected to the application of cyclic uniaxial strains of the magnitude of 10%. The modeling results predict the kinetics of the process of myogenic differentiation, including the number of cells in each stage of differentiation and the rates of differentiation from one stage to another for different strains from 4% to 16%. The developed model can help better understand the process of myogenic differentiation and the effects of mechanical cues on stem cell use in muscle therapies. Nature Publishing Group 2017-01-20 /pmc/articles/PMC5247743/ /pubmed/28106095 http://dx.doi.org/10.1038/srep40639 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Deshpande, Rajiv S. Spector, Alexander A. Modeling Stem Cell Myogenic Differentiation |
title | Modeling Stem Cell Myogenic Differentiation |
title_full | Modeling Stem Cell Myogenic Differentiation |
title_fullStr | Modeling Stem Cell Myogenic Differentiation |
title_full_unstemmed | Modeling Stem Cell Myogenic Differentiation |
title_short | Modeling Stem Cell Myogenic Differentiation |
title_sort | modeling stem cell myogenic differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247743/ https://www.ncbi.nlm.nih.gov/pubmed/28106095 http://dx.doi.org/10.1038/srep40639 |
work_keys_str_mv | AT deshpanderajivs modelingstemcellmyogenicdifferentiation AT spectoralexandera modelingstemcellmyogenicdifferentiation |