The β-globin Replicator greatly enhances the potential of S/MAR based episomal vectors for gene transfer into human haematopoietic progenitor cells

Specific human chromosomal elements enhance the performance of episomal gene-transfer vectors. S/MAR-based episomal vector pEPI-eGFP transfects CD34(+) haematopoietic cells, but only transiently. To address this issue we reinforced (1) transgene transcription by replacing the CMV promoter driving eG...

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Detalles Bibliográficos
Autores principales: Stavrou, Eleana F., Lazaris, Vassileios M., Giannakopoulos, Aristeidis, Papapetrou, Eirini, Spyridonidis, Alexandros, Zoumbos, Nikolas C., Gkountis, Antonis, Athanassiadou, Aglaia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247744/
https://www.ncbi.nlm.nih.gov/pubmed/28106085
http://dx.doi.org/10.1038/srep40673

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