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PGRMC1 Is a Novel Potential Tumor Biomarker of Human Renal Cell Carcinoma Based on Quantitative Proteomic and Integrative Biological Assessments

Progesterone receptor membrane component 1 (PGRMC1) is widely observed with an elevated level in multiple human cancers. However, the roles of PGRMC1 in renal cancer are not clear and merit further study. In this report, we made a systematic, integrative biological assessment for PGRMC1 in renal cel...

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Autores principales: Zhang, Dan, Xia, Xiangying, Wang, Xixi, Zhang, Peng, Lu, Weiliang, Yu, Yamei, Deng, Shi, Yang, Hanshuo, Zhu, Hongxia, Xu, Ningzhi, Liang, Shufang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249100/
https://www.ncbi.nlm.nih.gov/pubmed/28107520
http://dx.doi.org/10.1371/journal.pone.0170453
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author Zhang, Dan
Xia, Xiangying
Wang, Xixi
Zhang, Peng
Lu, Weiliang
Yu, Yamei
Deng, Shi
Yang, Hanshuo
Zhu, Hongxia
Xu, Ningzhi
Liang, Shufang
author_facet Zhang, Dan
Xia, Xiangying
Wang, Xixi
Zhang, Peng
Lu, Weiliang
Yu, Yamei
Deng, Shi
Yang, Hanshuo
Zhu, Hongxia
Xu, Ningzhi
Liang, Shufang
author_sort Zhang, Dan
collection PubMed
description Progesterone receptor membrane component 1 (PGRMC1) is widely observed with an elevated level in multiple human cancers. However, the roles of PGRMC1 in renal cancer are not clear and merit further study. In this report, we made a systematic, integrative biological assessment for PGRMC1 in renal cell carcinoma (RCC) by a quantitative proteomic identification, immunohistochemical detection, and its clinic pathologic significance analysis. We found that PGRMC1 abundance is increased by 3.91-fold in RCC tissues compared with its autologous para-cancerous tissues by a quantitative proteome identification. To validate the proteomic result with more confidence, 135 clinic RCC tissues were recruited to measure PGRMC1 abundance by immunohistochemical staining, and 63.7% RCC samples (n = 86) showed a higher abundance of PGRMC1 than the noncancerous counterparts. And the elevated PGRMC1 level was related to the tumor malignancy degree and overall survival of RCC patients. Meanwhile the average serum PGRMC1 concentration for RCC patients (n = 18) was significantly increased by 1.67 fold compared with healthy persons. Moreover an exogenous elevated abundance of PGRMC1 by plasmid transfections significantly enhanced cell proliferation of renal cancer cells in vitro. Our findings demonstrate PGRMC1, which promotes RCC progression phenotypes in vitro and in vivo, is a novel potential biomarker and therapeutic target for RCC.
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spelling pubmed-52491002017-02-06 PGRMC1 Is a Novel Potential Tumor Biomarker of Human Renal Cell Carcinoma Based on Quantitative Proteomic and Integrative Biological Assessments Zhang, Dan Xia, Xiangying Wang, Xixi Zhang, Peng Lu, Weiliang Yu, Yamei Deng, Shi Yang, Hanshuo Zhu, Hongxia Xu, Ningzhi Liang, Shufang PLoS One Research Article Progesterone receptor membrane component 1 (PGRMC1) is widely observed with an elevated level in multiple human cancers. However, the roles of PGRMC1 in renal cancer are not clear and merit further study. In this report, we made a systematic, integrative biological assessment for PGRMC1 in renal cell carcinoma (RCC) by a quantitative proteomic identification, immunohistochemical detection, and its clinic pathologic significance analysis. We found that PGRMC1 abundance is increased by 3.91-fold in RCC tissues compared with its autologous para-cancerous tissues by a quantitative proteome identification. To validate the proteomic result with more confidence, 135 clinic RCC tissues were recruited to measure PGRMC1 abundance by immunohistochemical staining, and 63.7% RCC samples (n = 86) showed a higher abundance of PGRMC1 than the noncancerous counterparts. And the elevated PGRMC1 level was related to the tumor malignancy degree and overall survival of RCC patients. Meanwhile the average serum PGRMC1 concentration for RCC patients (n = 18) was significantly increased by 1.67 fold compared with healthy persons. Moreover an exogenous elevated abundance of PGRMC1 by plasmid transfections significantly enhanced cell proliferation of renal cancer cells in vitro. Our findings demonstrate PGRMC1, which promotes RCC progression phenotypes in vitro and in vivo, is a novel potential biomarker and therapeutic target for RCC. Public Library of Science 2017-01-20 /pmc/articles/PMC5249100/ /pubmed/28107520 http://dx.doi.org/10.1371/journal.pone.0170453 Text en © 2017 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Dan
Xia, Xiangying
Wang, Xixi
Zhang, Peng
Lu, Weiliang
Yu, Yamei
Deng, Shi
Yang, Hanshuo
Zhu, Hongxia
Xu, Ningzhi
Liang, Shufang
PGRMC1 Is a Novel Potential Tumor Biomarker of Human Renal Cell Carcinoma Based on Quantitative Proteomic and Integrative Biological Assessments
title PGRMC1 Is a Novel Potential Tumor Biomarker of Human Renal Cell Carcinoma Based on Quantitative Proteomic and Integrative Biological Assessments
title_full PGRMC1 Is a Novel Potential Tumor Biomarker of Human Renal Cell Carcinoma Based on Quantitative Proteomic and Integrative Biological Assessments
title_fullStr PGRMC1 Is a Novel Potential Tumor Biomarker of Human Renal Cell Carcinoma Based on Quantitative Proteomic and Integrative Biological Assessments
title_full_unstemmed PGRMC1 Is a Novel Potential Tumor Biomarker of Human Renal Cell Carcinoma Based on Quantitative Proteomic and Integrative Biological Assessments
title_short PGRMC1 Is a Novel Potential Tumor Biomarker of Human Renal Cell Carcinoma Based on Quantitative Proteomic and Integrative Biological Assessments
title_sort pgrmc1 is a novel potential tumor biomarker of human renal cell carcinoma based on quantitative proteomic and integrative biological assessments
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249100/
https://www.ncbi.nlm.nih.gov/pubmed/28107520
http://dx.doi.org/10.1371/journal.pone.0170453
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