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Regulatory Mechanisms Underlying the Expression of Prolactin Receptor in Chicken Granulosa Cells

Prolactin (PRL) has both pro- and anti-gonadal roles in the regulation of avian ovarian functions through its interaction with the receptor (PRLR). However, neither the pattern of expression of PRLR nor its regulatory mechanisms during follicle development have been clearly defined. The objective of...

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Autores principales: Hu, Shenqiang, Duggavathi, Raj, Zadworny, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249103/
https://www.ncbi.nlm.nih.gov/pubmed/28107515
http://dx.doi.org/10.1371/journal.pone.0170409
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author Hu, Shenqiang
Duggavathi, Raj
Zadworny, David
author_facet Hu, Shenqiang
Duggavathi, Raj
Zadworny, David
author_sort Hu, Shenqiang
collection PubMed
description Prolactin (PRL) has both pro- and anti-gonadal roles in the regulation of avian ovarian functions through its interaction with the receptor (PRLR). However, neither the pattern of expression of PRLR nor its regulatory mechanisms during follicle development have been clearly defined. The objective of the present study was to investigate mechanisms of PRLR expression in chicken granulosa cells. Levels of PRLR transcript were highest in the stroma and walls of follicles < 2 mm in diameter and progressively declined with the maturation of follicles. In preovulatory follicles, PRLR was expressed at higher levels in granulosa than theca layers. FSH exerted the greatest stimulatory effect on PRLR and StAR expression in cultured granulosa cells of the 6–8 mm follicles but this effect declined as follicles matured to F1. In contrast, LH did not alter the expression of PRLR in granulosa cells of all follicular classes but increased levels of StAR in F2 and F1 granulosa cells. Both non-glycosylated- (NG-) and glycosylated- (G-) PRL upregulated basal PRLR expression in granulosa cells of the 6–8 mm, F3 or F1 follicles but had little effect in F2 follicles. Furthermore, FSH-stimulated PRLR expression was reduced by the addition of either isoform of PRL especially in F2 granulosa cells. These results indicate that PRLR is differentially distributed and regulated by FSH or PRL variants independently or in combination in the follicular hierarchy. By using activators and inhibitors, we further demonstrated that multiple signaling pathways, including PKA, PKC, PI3K, mTOR and AMPK, are not only directly involved in, but they can also converge to modulate ERK2 activity to regulate FSH-mediated PRLR and StAR expression in undifferentiated granulosa cells. These data provide new insights into the regulatory mechanisms controlling the expression of PRLR in granulosa cells.
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spelling pubmed-52491032017-02-06 Regulatory Mechanisms Underlying the Expression of Prolactin Receptor in Chicken Granulosa Cells Hu, Shenqiang Duggavathi, Raj Zadworny, David PLoS One Research Article Prolactin (PRL) has both pro- and anti-gonadal roles in the regulation of avian ovarian functions through its interaction with the receptor (PRLR). However, neither the pattern of expression of PRLR nor its regulatory mechanisms during follicle development have been clearly defined. The objective of the present study was to investigate mechanisms of PRLR expression in chicken granulosa cells. Levels of PRLR transcript were highest in the stroma and walls of follicles < 2 mm in diameter and progressively declined with the maturation of follicles. In preovulatory follicles, PRLR was expressed at higher levels in granulosa than theca layers. FSH exerted the greatest stimulatory effect on PRLR and StAR expression in cultured granulosa cells of the 6–8 mm follicles but this effect declined as follicles matured to F1. In contrast, LH did not alter the expression of PRLR in granulosa cells of all follicular classes but increased levels of StAR in F2 and F1 granulosa cells. Both non-glycosylated- (NG-) and glycosylated- (G-) PRL upregulated basal PRLR expression in granulosa cells of the 6–8 mm, F3 or F1 follicles but had little effect in F2 follicles. Furthermore, FSH-stimulated PRLR expression was reduced by the addition of either isoform of PRL especially in F2 granulosa cells. These results indicate that PRLR is differentially distributed and regulated by FSH or PRL variants independently or in combination in the follicular hierarchy. By using activators and inhibitors, we further demonstrated that multiple signaling pathways, including PKA, PKC, PI3K, mTOR and AMPK, are not only directly involved in, but they can also converge to modulate ERK2 activity to regulate FSH-mediated PRLR and StAR expression in undifferentiated granulosa cells. These data provide new insights into the regulatory mechanisms controlling the expression of PRLR in granulosa cells. Public Library of Science 2017-01-20 /pmc/articles/PMC5249103/ /pubmed/28107515 http://dx.doi.org/10.1371/journal.pone.0170409 Text en © 2017 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hu, Shenqiang
Duggavathi, Raj
Zadworny, David
Regulatory Mechanisms Underlying the Expression of Prolactin Receptor in Chicken Granulosa Cells
title Regulatory Mechanisms Underlying the Expression of Prolactin Receptor in Chicken Granulosa Cells
title_full Regulatory Mechanisms Underlying the Expression of Prolactin Receptor in Chicken Granulosa Cells
title_fullStr Regulatory Mechanisms Underlying the Expression of Prolactin Receptor in Chicken Granulosa Cells
title_full_unstemmed Regulatory Mechanisms Underlying the Expression of Prolactin Receptor in Chicken Granulosa Cells
title_short Regulatory Mechanisms Underlying the Expression of Prolactin Receptor in Chicken Granulosa Cells
title_sort regulatory mechanisms underlying the expression of prolactin receptor in chicken granulosa cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249103/
https://www.ncbi.nlm.nih.gov/pubmed/28107515
http://dx.doi.org/10.1371/journal.pone.0170409
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