Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H(2)O(2): Implications for Pharmacological Ascorbate Therapy

Cancer cell toxicity to therapeutic H(2)O(2) varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H(2)O(2) c...

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Autores principales: Erudaitius, Dieanira, Huang, Andrew, Kazmi, Sarah, Buettner, Garry R., Rodgers, Victor G. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249139/
https://www.ncbi.nlm.nih.gov/pubmed/28107421
http://dx.doi.org/10.1371/journal.pone.0170442
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author Erudaitius, Dieanira
Huang, Andrew
Kazmi, Sarah
Buettner, Garry R.
Rodgers, Victor G. J.
author_facet Erudaitius, Dieanira
Huang, Andrew
Kazmi, Sarah
Buettner, Garry R.
Rodgers, Victor G. J.
author_sort Erudaitius, Dieanira
collection PubMed
description Cancer cell toxicity to therapeutic H(2)O(2) varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H(2)O(2) concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H(2)O(2) doses (P < 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy.
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spelling pubmed-52491392017-02-06 Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H(2)O(2): Implications for Pharmacological Ascorbate Therapy Erudaitius, Dieanira Huang, Andrew Kazmi, Sarah Buettner, Garry R. Rodgers, Victor G. J. PLoS One Research Article Cancer cell toxicity to therapeutic H(2)O(2) varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H(2)O(2) concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H(2)O(2) doses (P < 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy. Public Library of Science 2017-01-20 /pmc/articles/PMC5249139/ /pubmed/28107421 http://dx.doi.org/10.1371/journal.pone.0170442 Text en © 2017 Erudaitius et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Erudaitius, Dieanira
Huang, Andrew
Kazmi, Sarah
Buettner, Garry R.
Rodgers, Victor G. J.
Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H(2)O(2): Implications for Pharmacological Ascorbate Therapy
title Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H(2)O(2): Implications for Pharmacological Ascorbate Therapy
title_full Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H(2)O(2): Implications for Pharmacological Ascorbate Therapy
title_fullStr Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H(2)O(2): Implications for Pharmacological Ascorbate Therapy
title_full_unstemmed Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H(2)O(2): Implications for Pharmacological Ascorbate Therapy
title_short Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H(2)O(2): Implications for Pharmacological Ascorbate Therapy
title_sort peroxiporin expression is an important factor for cancer cell susceptibility to therapeutic h(2)o(2): implications for pharmacological ascorbate therapy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249139/
https://www.ncbi.nlm.nih.gov/pubmed/28107421
http://dx.doi.org/10.1371/journal.pone.0170442
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