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Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice

Tiller number is one of the key factors that influences rice plant type and yield components. In this study, an EMS-induced rice tiller suppression mutant ts1 was characterized. Morphological and histological observations revealed that, in the ts1 plants, the tiller buds were abnormally formed and t...

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Autores principales: Liu, Lei, Meng, Fen, He, Yonggang, Zhu, Menghao, Shen, Yanhao, Zhang, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249193/
https://www.ncbi.nlm.nih.gov/pubmed/28107441
http://dx.doi.org/10.1371/journal.pone.0170574
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author Liu, Lei
Meng, Fen
He, Yonggang
Zhu, Menghao
Shen, Yanhao
Zhang, Zhihong
author_facet Liu, Lei
Meng, Fen
He, Yonggang
Zhu, Menghao
Shen, Yanhao
Zhang, Zhihong
author_sort Liu, Lei
collection PubMed
description Tiller number is one of the key factors that influences rice plant type and yield components. In this study, an EMS-induced rice tiller suppression mutant ts1 was characterized. Morphological and histological observations revealed that, in the ts1 plants, the tiller buds were abnormally formed and therefore cannot outgrow into tillers. With an F2 population derived from a cross between ts1 and an indica cultivar Wushansimiao, a major gene, tiller suppression 1 (ts1) was fine-mapped to a 108.5 kb genomic region between markers ID8378 and SSR6884 on the short arm of rice chromosome 2. Candidate gene analysis identified nineteen putative genes. Among them, ORF4 (LOC_Os02g01610) is a PPR gene which harbored a point mutation c.+733/C→T in ts1 mutant plants. A co-dominant SNP marker cd-733C/T was subsequently developed and the SNP assay demonstrated that the point mutation co-segregated with tiller suppression phenotype. Quantitative RT-PCR analysis showed that the expression level of ORF4 in ts1 plants was significantly lower than that in their wild plants, and the expression of rice tillering regulators MOC1 and HTD1 was also significantly decreased in ts1 plants. Our data indicated that ORF4 was a strong candidate gene for ts1 and ts1 might play a role in regulating rice tillering through MOC1 and HTD1 associated pathway. The results above provide a basis for further functional characterization of ts1 and will shed light on molecular mechanism of rice tillering. The informative SNP marker cd-733C/T will facilitate marker-assisted selection of ts1 in rice plant type breeding.
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spelling pubmed-52491932017-02-06 Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice Liu, Lei Meng, Fen He, Yonggang Zhu, Menghao Shen, Yanhao Zhang, Zhihong PLoS One Research Article Tiller number is one of the key factors that influences rice plant type and yield components. In this study, an EMS-induced rice tiller suppression mutant ts1 was characterized. Morphological and histological observations revealed that, in the ts1 plants, the tiller buds were abnormally formed and therefore cannot outgrow into tillers. With an F2 population derived from a cross between ts1 and an indica cultivar Wushansimiao, a major gene, tiller suppression 1 (ts1) was fine-mapped to a 108.5 kb genomic region between markers ID8378 and SSR6884 on the short arm of rice chromosome 2. Candidate gene analysis identified nineteen putative genes. Among them, ORF4 (LOC_Os02g01610) is a PPR gene which harbored a point mutation c.+733/C→T in ts1 mutant plants. A co-dominant SNP marker cd-733C/T was subsequently developed and the SNP assay demonstrated that the point mutation co-segregated with tiller suppression phenotype. Quantitative RT-PCR analysis showed that the expression level of ORF4 in ts1 plants was significantly lower than that in their wild plants, and the expression of rice tillering regulators MOC1 and HTD1 was also significantly decreased in ts1 plants. Our data indicated that ORF4 was a strong candidate gene for ts1 and ts1 might play a role in regulating rice tillering through MOC1 and HTD1 associated pathway. The results above provide a basis for further functional characterization of ts1 and will shed light on molecular mechanism of rice tillering. The informative SNP marker cd-733C/T will facilitate marker-assisted selection of ts1 in rice plant type breeding. Public Library of Science 2017-01-20 /pmc/articles/PMC5249193/ /pubmed/28107441 http://dx.doi.org/10.1371/journal.pone.0170574 Text en © 2017 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Liu, Lei
Meng, Fen
He, Yonggang
Zhu, Menghao
Shen, Yanhao
Zhang, Zhihong
Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice
title Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice
title_full Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice
title_fullStr Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice
title_full_unstemmed Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice
title_short Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice
title_sort fine mapping and candidate gene analysis of the tiller suppression gene ts1 in rice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249193/
https://www.ncbi.nlm.nih.gov/pubmed/28107441
http://dx.doi.org/10.1371/journal.pone.0170574
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