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Reversible Oligonucleotide Chain Blocking Enables Bead Capture and Amplification of T-Cell Receptor α and β Chain mRNAs
[Image: see text] Next-generation sequencing (NGS) has proven to be an exceptionally powerful tool for studying genetic variation and differences in gene expression profiles between cell populations. However, these population-wide studies are limited by their inability to detect variation between in...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249220/ https://www.ncbi.nlm.nih.gov/pubmed/27478996 http://dx.doi.org/10.1021/jacs.6b04465 |
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author | Hanson, W. Miachel Chen, Zhe Jackson, Laurie K. Attaf, Meriem Sewell, Andrew K. Heemstra, Jennifer M. Phillips, John D. |
author_facet | Hanson, W. Miachel Chen, Zhe Jackson, Laurie K. Attaf, Meriem Sewell, Andrew K. Heemstra, Jennifer M. Phillips, John D. |
author_sort | Hanson, W. Miachel |
collection | PubMed |
description | [Image: see text] Next-generation sequencing (NGS) has proven to be an exceptionally powerful tool for studying genetic variation and differences in gene expression profiles between cell populations. However, these population-wide studies are limited by their inability to detect variation between individual cells within a population, inspiring the development of single-cell techniques such as Drop-seq, which add a unique barcode to the mRNA from each cell prior to sequencing. Current Drop-seq technology enables capture, amplification, and barcoding of the entire mRNA transcriptome of individual cells. NGS can then be used to sequence the 3′-end of each message to build a cell-specific transcriptional landscape. However, current technology does not allow high-throughput capture of information distant from the mRNA poly-A tail. Thus, gene profiling would have much greater utility if beads could be generated having multiple transcript-specific capture sequences. Here we report the use of a reversible chain blocking group to enable synthesis of DNA barcoded beads having capture sequences for the constant domains of the T-cell receptor α and β chain mRNAs. We demonstrate that these beads can be used to capture and pair TCRα and TCRβ sequences from total T-cell RNA, enabling reverse transcription and PCR amplification of these sequences. This is the first example of capture beads having more than one capture sequence, and we envision that this technology will be of high utility for applications such as pairing the antigen receptor chains that give rise to autoimmune diseases or measuring the ratios of mRNA splice variants in cancer stem cells. |
format | Online Article Text |
id | pubmed-5249220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-52492202017-01-24 Reversible Oligonucleotide Chain Blocking Enables Bead Capture and Amplification of T-Cell Receptor α and β Chain mRNAs Hanson, W. Miachel Chen, Zhe Jackson, Laurie K. Attaf, Meriem Sewell, Andrew K. Heemstra, Jennifer M. Phillips, John D. J Am Chem Soc [Image: see text] Next-generation sequencing (NGS) has proven to be an exceptionally powerful tool for studying genetic variation and differences in gene expression profiles between cell populations. However, these population-wide studies are limited by their inability to detect variation between individual cells within a population, inspiring the development of single-cell techniques such as Drop-seq, which add a unique barcode to the mRNA from each cell prior to sequencing. Current Drop-seq technology enables capture, amplification, and barcoding of the entire mRNA transcriptome of individual cells. NGS can then be used to sequence the 3′-end of each message to build a cell-specific transcriptional landscape. However, current technology does not allow high-throughput capture of information distant from the mRNA poly-A tail. Thus, gene profiling would have much greater utility if beads could be generated having multiple transcript-specific capture sequences. Here we report the use of a reversible chain blocking group to enable synthesis of DNA barcoded beads having capture sequences for the constant domains of the T-cell receptor α and β chain mRNAs. We demonstrate that these beads can be used to capture and pair TCRα and TCRβ sequences from total T-cell RNA, enabling reverse transcription and PCR amplification of these sequences. This is the first example of capture beads having more than one capture sequence, and we envision that this technology will be of high utility for applications such as pairing the antigen receptor chains that give rise to autoimmune diseases or measuring the ratios of mRNA splice variants in cancer stem cells. American Chemical Society 2016-08-01 2016-09-07 /pmc/articles/PMC5249220/ /pubmed/27478996 http://dx.doi.org/10.1021/jacs.6b04465 Text en Copyright © 2016 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Hanson, W. Miachel Chen, Zhe Jackson, Laurie K. Attaf, Meriem Sewell, Andrew K. Heemstra, Jennifer M. Phillips, John D. Reversible Oligonucleotide Chain Blocking Enables Bead Capture and Amplification of T-Cell Receptor α and β Chain mRNAs |
title | Reversible
Oligonucleotide Chain Blocking Enables
Bead Capture and Amplification of T-Cell Receptor α and
β Chain mRNAs |
title_full | Reversible
Oligonucleotide Chain Blocking Enables
Bead Capture and Amplification of T-Cell Receptor α and
β Chain mRNAs |
title_fullStr | Reversible
Oligonucleotide Chain Blocking Enables
Bead Capture and Amplification of T-Cell Receptor α and
β Chain mRNAs |
title_full_unstemmed | Reversible
Oligonucleotide Chain Blocking Enables
Bead Capture and Amplification of T-Cell Receptor α and
β Chain mRNAs |
title_short | Reversible
Oligonucleotide Chain Blocking Enables
Bead Capture and Amplification of T-Cell Receptor α and
β Chain mRNAs |
title_sort | reversible
oligonucleotide chain blocking enables
bead capture and amplification of t-cell receptor α and
β chain mrnas |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249220/ https://www.ncbi.nlm.nih.gov/pubmed/27478996 http://dx.doi.org/10.1021/jacs.6b04465 |
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