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Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats

Hydrogen sulfide (H(2)S) has been shown to protect the gastric mucosa through several protective mechanisms but till now its effect on mRNA expression of sodium bicarbonate cotransporter 1 (NBC1), trefoil factor1 (TFF1) and trefoil factor2 (TFF2) was not investigated. This study was aimed to evaluat...

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Autores principales: Cheraghi, Parisa, Mard, Seyyed Ali, Nagi, Tahereh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5251355/
https://www.ncbi.nlm.nih.gov/pubmed/28144424
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author Cheraghi, Parisa
Mard, Seyyed Ali
Nagi, Tahereh
author_facet Cheraghi, Parisa
Mard, Seyyed Ali
Nagi, Tahereh
author_sort Cheraghi, Parisa
collection PubMed
description Hydrogen sulfide (H(2)S) has been shown to protect the gastric mucosa through several protective mechanisms but till now its effect on mRNA expression of sodium bicarbonate cotransporter 1 (NBC1), trefoil factor1 (TFF1) and trefoil factor2 (TFF2) was not investigated. This study was aimed to evaluate the effect of H(2)S on mRNA expression of NBC1, TFF1 and TFF2 in rat gastric mucosa in response to gastric distention. Thirty two rats were randomly assigned into four equal groups. They were control (C), distention (D), propargylglycine (PAG)-, and NaHS-treated groups. To evaluate the effect of exogenous and endogenous H(2)S on gene expression of NBC1, TFF1 and TFF2, two groups of rats were received H(2)S donor, intra-peritoneal NaHS (80 µg Kg(-1)), and PAG (50 mg kg(-1)), accompanied to stimulate the gastric acid secretion, respectively. Under general anesthesia and laparotomy, a catheter was inserted into the stomach through duodenum for instillation of isotonic saline for gastric distention. Ninety min after beginning the experiment, animals were sacrificed and the gastric mucosa was collected to determine total acid content of gastric effluents and to quantify the mRNA expression of studied genes by quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that A) gastric distention increased the level of mRNA expressions of NBC1, TFF1 and TFF2; B) these levels in NaHS-treated rats were significantly higher than those in Distention group; and C) PAG decreased the expression levels of NBC1 and TFF1. The Findings showed H(2)S upregulated gene expression of NBC1, TFF1 and TFF2 in gastric mucosa.
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spelling pubmed-52513552017-01-31 Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats Cheraghi, Parisa Mard, Seyyed Ali Nagi, Tahereh Vet Res Forum Original Article Hydrogen sulfide (H(2)S) has been shown to protect the gastric mucosa through several protective mechanisms but till now its effect on mRNA expression of sodium bicarbonate cotransporter 1 (NBC1), trefoil factor1 (TFF1) and trefoil factor2 (TFF2) was not investigated. This study was aimed to evaluate the effect of H(2)S on mRNA expression of NBC1, TFF1 and TFF2 in rat gastric mucosa in response to gastric distention. Thirty two rats were randomly assigned into four equal groups. They were control (C), distention (D), propargylglycine (PAG)-, and NaHS-treated groups. To evaluate the effect of exogenous and endogenous H(2)S on gene expression of NBC1, TFF1 and TFF2, two groups of rats were received H(2)S donor, intra-peritoneal NaHS (80 µg Kg(-1)), and PAG (50 mg kg(-1)), accompanied to stimulate the gastric acid secretion, respectively. Under general anesthesia and laparotomy, a catheter was inserted into the stomach through duodenum for instillation of isotonic saline for gastric distention. Ninety min after beginning the experiment, animals were sacrificed and the gastric mucosa was collected to determine total acid content of gastric effluents and to quantify the mRNA expression of studied genes by quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that A) gastric distention increased the level of mRNA expressions of NBC1, TFF1 and TFF2; B) these levels in NaHS-treated rats were significantly higher than those in Distention group; and C) PAG decreased the expression levels of NBC1 and TFF1. The Findings showed H(2)S upregulated gene expression of NBC1, TFF1 and TFF2 in gastric mucosa. Urmia University Press 2016 2016-12-15 /pmc/articles/PMC5251355/ /pubmed/28144424 Text en © 2016 Urmia University. All rights reserved. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Cheraghi, Parisa
Mard, Seyyed Ali
Nagi, Tahereh
Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats
title Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats
title_full Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats
title_fullStr Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats
title_full_unstemmed Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats
title_short Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats
title_sort hydrogen sulfide upregulated mrna expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5251355/
https://www.ncbi.nlm.nih.gov/pubmed/28144424
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AT nagitahereh hydrogensulfideupregulatedmrnaexpressionsofsodiumbicarbonatecotransporter1trefoilfactor1andtrefoilfactor2ingastricmucosainrats