An Efficient Electroporation Protocol for the Genetic Modification of Mammalian Cells

Genetic modification of cell lines and primary cells is an expensive and cumbersome approach, often involving the use of viral vectors. Electroporation using square-wave generating devices, like Lonza’s Nucleofector, is a widely used option, but the costs associated with the acquisition of electropo...

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Detalles Bibliográficos
Autores principales: Chicaybam, Leonardo, Barcelos, Camila, Peixoto, Barbara, Carneiro, Mayra, Limia, Cintia Gomez, Redondo, Patrícia, Lira, Carla, Paraguassú-Braga, Flávio, Vasconcelos, Zilton Farias Meira De, Barros, Luciana, Bonamino, Martin Hernán
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5253374/
https://www.ncbi.nlm.nih.gov/pubmed/28168187
http://dx.doi.org/10.3389/fbioe.2016.00099

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