CRISPR‐Cas9‐mediated efficient directed mutagenesis and RAD51‐dependent and RAD51‐independent gene targeting in the moss Physcomitrella patens

The ability to address the CRISPR‐Cas9 nuclease complex to any target DNA using customizable single‐guide RNAs has now permitted genome engineering in many species. Here, we report its first successful use in a nonvascular plant, the moss Physcomitrella patens. Single‐guide RNAs (sgRNAs) were design...

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Detalles Bibliográficos
Autores principales: Collonnier, Cécile, Epert, Aline, Mara, Kostlend, Maclot, François, Guyon‐Debast, Anouchka, Charlot, Florence, White, Charles, Schaefer, Didier G., Nogué, Fabien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5253467/
https://www.ncbi.nlm.nih.gov/pubmed/27368642
http://dx.doi.org/10.1111/pbi.12596

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