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Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses

The assembly of the first centrosome occurs upon fertilisation when male centrioles recruit pericentriolar material (PCM) from the egg cytoplasm. The mechanisms underlying the proper assembly of centrosomes during early embryogenesis remain obscure. We identify Wdr8 as a novel maternally essential p...

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Autores principales: Inoue, Daigo, Stemmer, Manuel, Thumberger, Thomas, Ruppert, Thomas, Bärenz, Felix, Wittbrodt, Joachim, Gruss, Oliver J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5253655/
https://www.ncbi.nlm.nih.gov/pubmed/28098238
http://dx.doi.org/10.1038/ncomms14090
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author Inoue, Daigo
Stemmer, Manuel
Thumberger, Thomas
Ruppert, Thomas
Bärenz, Felix
Wittbrodt, Joachim
Gruss, Oliver J.
author_facet Inoue, Daigo
Stemmer, Manuel
Thumberger, Thomas
Ruppert, Thomas
Bärenz, Felix
Wittbrodt, Joachim
Gruss, Oliver J.
author_sort Inoue, Daigo
collection PubMed
description The assembly of the first centrosome occurs upon fertilisation when male centrioles recruit pericentriolar material (PCM) from the egg cytoplasm. The mechanisms underlying the proper assembly of centrosomes during early embryogenesis remain obscure. We identify Wdr8 as a novel maternally essential protein that is required for centrosome assembly during embryonic mitoses of medaka (Oryzias latipes). By CRISPR–Cas9-mediated knockout, maternal/zygotic Wdr8-null (m/zWdr8(−/−)) blastomeres exhibit severe defects in centrosome structure that lead to asymmetric division, multipolar mitotic spindles and chromosome alignment errors. Via its WD40 domains, Wdr8 interacts with the centriolar satellite protein SSX2IP. Combining targeted gene knockout and in vivo reconstitution of the maternally essential Wdr8–SSX2IP complex reveals an essential link between maternal centrosome proteins and the stability of the zygotic genome for accurate vertebrate embryogenesis. Our approach provides a way of distinguishing maternal from paternal effects in early embryos and should contribute to understanding molecular defects in human infertility.
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spelling pubmed-52536552017-02-03 Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses Inoue, Daigo Stemmer, Manuel Thumberger, Thomas Ruppert, Thomas Bärenz, Felix Wittbrodt, Joachim Gruss, Oliver J. Nat Commun Article The assembly of the first centrosome occurs upon fertilisation when male centrioles recruit pericentriolar material (PCM) from the egg cytoplasm. The mechanisms underlying the proper assembly of centrosomes during early embryogenesis remain obscure. We identify Wdr8 as a novel maternally essential protein that is required for centrosome assembly during embryonic mitoses of medaka (Oryzias latipes). By CRISPR–Cas9-mediated knockout, maternal/zygotic Wdr8-null (m/zWdr8(−/−)) blastomeres exhibit severe defects in centrosome structure that lead to asymmetric division, multipolar mitotic spindles and chromosome alignment errors. Via its WD40 domains, Wdr8 interacts with the centriolar satellite protein SSX2IP. Combining targeted gene knockout and in vivo reconstitution of the maternally essential Wdr8–SSX2IP complex reveals an essential link between maternal centrosome proteins and the stability of the zygotic genome for accurate vertebrate embryogenesis. Our approach provides a way of distinguishing maternal from paternal effects in early embryos and should contribute to understanding molecular defects in human infertility. Nature Publishing Group 2017-01-18 /pmc/articles/PMC5253655/ /pubmed/28098238 http://dx.doi.org/10.1038/ncomms14090 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Inoue, Daigo
Stemmer, Manuel
Thumberger, Thomas
Ruppert, Thomas
Bärenz, Felix
Wittbrodt, Joachim
Gruss, Oliver J.
Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses
title Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses
title_full Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses
title_fullStr Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses
title_full_unstemmed Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses
title_short Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses
title_sort expression of the novel maternal centrosome assembly factor wdr8 is required for vertebrate embryonic mitoses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5253655/
https://www.ncbi.nlm.nih.gov/pubmed/28098238
http://dx.doi.org/10.1038/ncomms14090
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