Cargando…
Carbon translocation from a plant to an insect-pathogenic endophytic fungus
Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen. Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5253661/ https://www.ncbi.nlm.nih.gov/pubmed/28098142 http://dx.doi.org/10.1038/ncomms14245 |
_version_ | 1782498203693744128 |
---|---|
author | Behie, Scott W. Moreira, Camila C. Sementchoukova, Irina Barelli, Larissa Zelisko, Paul M. Bidochka, Michael J. |
author_facet | Behie, Scott W. Moreira, Camila C. Sementchoukova, Irina Barelli, Larissa Zelisko, Paul M. Bidochka, Michael J. |
author_sort | Behie, Scott W. |
collection | PubMed |
description | Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen. Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia. We speculated that in exchange for this insect-derived nitrogen, the plant would provide photosynthate to the fungus. By using (13)CO(2), we show the incorporation of (13)C into photosynthate and the subsequent translocation of (13)C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex. We determined the amount of (13)C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy. These findings are evidence that the host plant is providing photosynthate to the fungus, likely in exchange for insect-derived nitrogen in a tripartite, and symbiotic, interaction. |
format | Online Article Text |
id | pubmed-5253661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52536612017-02-03 Carbon translocation from a plant to an insect-pathogenic endophytic fungus Behie, Scott W. Moreira, Camila C. Sementchoukova, Irina Barelli, Larissa Zelisko, Paul M. Bidochka, Michael J. Nat Commun Article Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen. Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia. We speculated that in exchange for this insect-derived nitrogen, the plant would provide photosynthate to the fungus. By using (13)CO(2), we show the incorporation of (13)C into photosynthate and the subsequent translocation of (13)C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex. We determined the amount of (13)C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy. These findings are evidence that the host plant is providing photosynthate to the fungus, likely in exchange for insect-derived nitrogen in a tripartite, and symbiotic, interaction. Nature Publishing Group 2017-01-18 /pmc/articles/PMC5253661/ /pubmed/28098142 http://dx.doi.org/10.1038/ncomms14245 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Behie, Scott W. Moreira, Camila C. Sementchoukova, Irina Barelli, Larissa Zelisko, Paul M. Bidochka, Michael J. Carbon translocation from a plant to an insect-pathogenic endophytic fungus |
title | Carbon translocation from a plant to an insect-pathogenic endophytic fungus |
title_full | Carbon translocation from a plant to an insect-pathogenic endophytic fungus |
title_fullStr | Carbon translocation from a plant to an insect-pathogenic endophytic fungus |
title_full_unstemmed | Carbon translocation from a plant to an insect-pathogenic endophytic fungus |
title_short | Carbon translocation from a plant to an insect-pathogenic endophytic fungus |
title_sort | carbon translocation from a plant to an insect-pathogenic endophytic fungus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5253661/ https://www.ncbi.nlm.nih.gov/pubmed/28098142 http://dx.doi.org/10.1038/ncomms14245 |
work_keys_str_mv | AT behiescottw carbontranslocationfromaplanttoaninsectpathogenicendophyticfungus AT moreiracamilac carbontranslocationfromaplanttoaninsectpathogenicendophyticfungus AT sementchoukovairina carbontranslocationfromaplanttoaninsectpathogenicendophyticfungus AT barellilarissa carbontranslocationfromaplanttoaninsectpathogenicendophyticfungus AT zeliskopaulm carbontranslocationfromaplanttoaninsectpathogenicendophyticfungus AT bidochkamichaelj carbontranslocationfromaplanttoaninsectpathogenicendophyticfungus |