Cargando…

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

Substantial efforts are being made to optimize the CRISPR/Cas9 system for precision crop breeding. The avoidance of transgene integration and reduction of off-target mutations are the most important targets for optimization. Here, we describe an efficient genome editing method for bread wheat using...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liang, Zhen, Chen, Kunling, Li, Tingdong, Zhang, Yi, Wang, Yanpeng, Zhao, Qian, Liu, Jinxing, Zhang, Huawei, Liu, Cuimin, Ran, Yidong, Gao, Caixia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5253684/ https://www.ncbi.nlm.nih.gov/pubmed/28098143 http://dx.doi.org/10.1038/ncomms14261

Ejemplares similares

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
por: Zhang, Yi, et al.
Publicado: (2016)

Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
por: Zhang, Song, et al.
Publicado: (2021)

Genotyping genome‐edited mutations in plants using CRISPR ribonucleoprotein complexes
por: Liang, Zhen, et al.
Publicado: (2018)

Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases
por: Zhang, Dingbo, et al.
Publicado: (2017)

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
por: Svitashev, Sergei, et al.
Publicado: (2016)

In Vitro Pre-validation of Gene Editing by CRISPR/Cas9 Ribonucleoprotein
por: Mehravar, Maryam, et al.
Publicado: (2019)

Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana
por: Wu, Shaoping, et al.
Publicado: (2020)

CRISPR-Cas9 ribonucleoprotein-mediated co-editing and counterselection in the rice blast fungus
por: Foster, Andrew J., et al.
Publicado: (2018)

CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Primary Innate Immune Cells
por: Hildreth, Andrew D., et al.
Publicado: (2020)

Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum
por: Lee, Nahyun, et al.
Publicado: (2022)

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein
por: Bevacqua, Romina J., et al.
Publicado: (2023)

Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing
por: Fu, Ya-Wen, et al.
Publicado: (2021)

Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
por: Liu, Qizhi, et al.
Publicado: (2018)

DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins
por: Malnoy, Mickael, et al.
Publicado: (2016)

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
por: Chaverra-Rodriguez, Duverney, et al.
Publicado: (2018)

PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
por: Sant’Ana, Rodrigo Ribeiro Arnt, et al.
Publicado: (2020)

Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing
por: Wei, Tuo, et al.
Publicado: (2020)

Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method
por: Yu, Lu, et al.
Publicado: (2022)

Genome editing in Penicillium funiculosum using in vitro assembled CRISPR-Cas9 ribonucleoprotein complexes
por: Sinha, Tulika, et al.
Publicado: (2022)

Efficient multiplex genome editing by CRISPR/Cas9 in common wheat
por: Li, Jihu, et al.
Publicado: (2020)

Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
por: Li, Chao, et al.
Publicado: (2018)

CRISPR/Cas9 Ribonucleoprotein-Based Genome Editing Methodology in the Marine Protozoan Parasite Perkinsus marinus
por: Yadavalli, Raghavendra, et al.
Publicado: (2021)

Carrier-Free Cellular Transport of CRISPR/Cas9 Ribonucleoprotein for Genome Editing by Cold Atmospheric Plasma
por: Cui, Haodong, et al.
Publicado: (2021)

CRISPR/Cas9‐mediated genome editing for wheat grain quality improvement
por: Zhang, Shujuan, et al.
Publicado: (2021)

High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
por: Paix, Alexandre, et al.
Publicado: (2015)

Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins
por: Soares Medeiros, Lia Carolina, et al.
Publicado: (2017)

A DNA-Free Editing Platform for Genetic Screens in Soybean via CRISPR/Cas9 Ribonucleoprotein Delivery
por: Subburaj, Saminathan, et al.
Publicado: (2022)

DNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration
por: Najafi, Samaneh, et al.
Publicado: (2022)

The sonication-assisted whisker method enables CRISPR-Cas9 ribonucleoprotein delivery to induce genome editing in rice
por: Nakamura, Akiyoshi, et al.
Publicado: (2023)

Efficient Genome Editing in Setaria italica Using CRISPR/Cas9 and Base Editors
por: Liang, Zhen, et al.
Publicado: (2022)

Development of an Agrobacterium‐delivered CRISPR/Cas9 system for wheat genome editing
por: Zhang, Zhengzhi, et al.
Publicado: (2019)

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
por: Farboud, Behnom, et al.
Publicado: (2018)

CRISPR/Cas9 ribonucleoprotein (RNP) complex enables higher viability of transfected cells in genome editing of acute myeloid cells
por: Cheng, Qinquan, et al.
Publicado: (2022)

DNA-Free Genome Editing of Brassica oleracea and B. rapa Protoplasts Using CRISPR-Cas9 Ribonucleoprotein Complexes
por: Murovec, Jana, et al.
Publicado: (2018)

Efficient genome editing in filamentous fungi via an improved CRISPR‐Cas9 ribonucleoprotein method facilitated by chemical reagents
por: Zou, Gen, et al.
Publicado: (2020)

Generation of novel self‐incompatible Brassica napus by CRISPR/Cas9
por: Dou, Shengwei, et al.
Publicado: (2021)

Production of Conjoined Transgenic and Edited Barley and Wheat Plants for Nud Genes Using the CRISPR/SpCas9 System
por: Zang, Yiming, et al.
Publicado: (2022)

gRNA validation for wheat genome editing with the CRISPR-Cas9 system
por: Arndell, Taj, et al.
Publicado: (2019)

CRISPR-Cas9 Gene Editing of the Sal1 Gene Family in Wheat
por: Mohr, Toni, et al.
Publicado: (2022)

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene
por: Banakar, Raviraj, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_hkviav7hg1qnt2i24qrb27sfl9