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Multi-omics analyses reveal metabolic alterations regulated by hepatitis B virus core protein in hepatocellular carcinoma cells
Chronic hepatitis B virus (HBV) infection is partly responsible for hepatitis, fatty liver disease and hepatocellular carcinoma (HCC). HBV core protein (HBc), encoded by the HBV genome, may play a significant role in HBV life cycle. However, the function of HBc in the occurrence and development of l...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5253728/ https://www.ncbi.nlm.nih.gov/pubmed/28112229 http://dx.doi.org/10.1038/srep41089 |
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author | Xie, Qi Fan, Fengxu Wei, Wei Liu, Yang Xu, Zhongwei Zhai, Linhui Qi, Yingzi Ye, Bingyu Zhang, Yao Basu, Sumit Zhao, Zhihu Wu, Junzhu Xu, Ping |
author_facet | Xie, Qi Fan, Fengxu Wei, Wei Liu, Yang Xu, Zhongwei Zhai, Linhui Qi, Yingzi Ye, Bingyu Zhang, Yao Basu, Sumit Zhao, Zhihu Wu, Junzhu Xu, Ping |
author_sort | Xie, Qi |
collection | PubMed |
description | Chronic hepatitis B virus (HBV) infection is partly responsible for hepatitis, fatty liver disease and hepatocellular carcinoma (HCC). HBV core protein (HBc), encoded by the HBV genome, may play a significant role in HBV life cycle. However, the function of HBc in the occurrence and development of liver disease is still unclear. To investigate the underlying mechanisms, HBc-transfected HCC cells were characterized by multi-omics analyses. Combining proteomics and metabolomics analyses, our results showed that HBc promoted the expression of metabolic enzymes and the secretion of metabolites in HCC cells. In addition, glycolysis and amino acid metabolism were significantly up-regulated by HBc. Moreover, Max-like protein X (MLX) might be recruited and enriched by HBc in the nucleus to regulate glycolysis pathways. This study provides further insights into the function of HBc in the molecular pathogenesis of HBV-induced diseases and indicates that metabolic reprogramming appears to be a hallmark of HBc transfection. |
format | Online Article Text |
id | pubmed-5253728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52537282017-01-24 Multi-omics analyses reveal metabolic alterations regulated by hepatitis B virus core protein in hepatocellular carcinoma cells Xie, Qi Fan, Fengxu Wei, Wei Liu, Yang Xu, Zhongwei Zhai, Linhui Qi, Yingzi Ye, Bingyu Zhang, Yao Basu, Sumit Zhao, Zhihu Wu, Junzhu Xu, Ping Sci Rep Article Chronic hepatitis B virus (HBV) infection is partly responsible for hepatitis, fatty liver disease and hepatocellular carcinoma (HCC). HBV core protein (HBc), encoded by the HBV genome, may play a significant role in HBV life cycle. However, the function of HBc in the occurrence and development of liver disease is still unclear. To investigate the underlying mechanisms, HBc-transfected HCC cells were characterized by multi-omics analyses. Combining proteomics and metabolomics analyses, our results showed that HBc promoted the expression of metabolic enzymes and the secretion of metabolites in HCC cells. In addition, glycolysis and amino acid metabolism were significantly up-regulated by HBc. Moreover, Max-like protein X (MLX) might be recruited and enriched by HBc in the nucleus to regulate glycolysis pathways. This study provides further insights into the function of HBc in the molecular pathogenesis of HBV-induced diseases and indicates that metabolic reprogramming appears to be a hallmark of HBc transfection. Nature Publishing Group 2017-01-23 /pmc/articles/PMC5253728/ /pubmed/28112229 http://dx.doi.org/10.1038/srep41089 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Xie, Qi Fan, Fengxu Wei, Wei Liu, Yang Xu, Zhongwei Zhai, Linhui Qi, Yingzi Ye, Bingyu Zhang, Yao Basu, Sumit Zhao, Zhihu Wu, Junzhu Xu, Ping Multi-omics analyses reveal metabolic alterations regulated by hepatitis B virus core protein in hepatocellular carcinoma cells |
title | Multi-omics analyses reveal metabolic alterations regulated by hepatitis B virus core protein in hepatocellular carcinoma cells |
title_full | Multi-omics analyses reveal metabolic alterations regulated by hepatitis B virus core protein in hepatocellular carcinoma cells |
title_fullStr | Multi-omics analyses reveal metabolic alterations regulated by hepatitis B virus core protein in hepatocellular carcinoma cells |
title_full_unstemmed | Multi-omics analyses reveal metabolic alterations regulated by hepatitis B virus core protein in hepatocellular carcinoma cells |
title_short | Multi-omics analyses reveal metabolic alterations regulated by hepatitis B virus core protein in hepatocellular carcinoma cells |
title_sort | multi-omics analyses reveal metabolic alterations regulated by hepatitis b virus core protein in hepatocellular carcinoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5253728/ https://www.ncbi.nlm.nih.gov/pubmed/28112229 http://dx.doi.org/10.1038/srep41089 |
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