Simplified detection of the hybridized DNA using a graphene field effect transistor

Detection of disease-related gene expression by DNA hybridization is a useful diagnostic method. In this study a monolayer graphene field effect transistor (GFET) was fabricated for the detection of a particular single-stranded DNA (target DNA). The probe DNA, which is a single-stranded DNA with a complementary nucleotide sequence, was directly immobilized onto the graphene surface without any linker. The V(Dirac) was shifted to the negative direction in the probe DNA immobilization. A further shift of V(Dirac) in the negative direction was observed when the target DNA was applied to GFET, but no shift was observed upon the application of non-complementary mismatched DNA. Direct immobilization of double-stranded DNA onto the graphene surface also shifted the V(Dirac) in the negative direction to the same extent as that of the shift induced by the immobilization of probe DNA and following target DNA application. These results suggest that the further shift of V(Dirac) after application of the target DNA to the GFET was caused by the hybridization between the probe DNA and target DNA.