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Design of an expression system to enhance MBP-mediated crystallization
Crystallization chaperones have been used to facilitate the crystallization of challenging proteins. Even though the maltose-binding protein (MBP) is one of the most commonly used crystallization chaperones, the design of optimal expression constructs for crystallization of MBP fusion proteins remai...
Autores principales: | Jin, Tengchuan, Chuenchor, Watchalee, Jiang, Jiansheng, Cheng, Jinbo, Li, Yajuan, Fang, Kang, Huang, Mo, Smith, Patrick, Xiao, Tsan Sam |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5256280/ https://www.ncbi.nlm.nih.gov/pubmed/28112203 http://dx.doi.org/10.1038/srep40991 |
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