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FRNA Bacteriophages as Viral Indicators of Faecal Contamination in Mexican Tropical Aquatic Systems

A particular challenge to water safety in populous intertropical regions is the lack of reliable faecal indicators to detect microbiological contamination of water, while the numerical relationships of specific viral indicators remain largely unexplored. The aim of this study was to investigate the...

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Autores principales: Arredondo-Hernandez, Luis Jose Rene, Diaz-Avalos, Carlos, Lopez-Vidal, Yolanda, Castillo-Rojas, Gonzalo, Mazari-Hiriart, Marisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5256921/
https://www.ncbi.nlm.nih.gov/pubmed/28114378
http://dx.doi.org/10.1371/journal.pone.0170399
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author Arredondo-Hernandez, Luis Jose Rene
Diaz-Avalos, Carlos
Lopez-Vidal, Yolanda
Castillo-Rojas, Gonzalo
Mazari-Hiriart, Marisa
author_facet Arredondo-Hernandez, Luis Jose Rene
Diaz-Avalos, Carlos
Lopez-Vidal, Yolanda
Castillo-Rojas, Gonzalo
Mazari-Hiriart, Marisa
author_sort Arredondo-Hernandez, Luis Jose Rene
collection PubMed
description A particular challenge to water safety in populous intertropical regions is the lack of reliable faecal indicators to detect microbiological contamination of water, while the numerical relationships of specific viral indicators remain largely unexplored. The aim of this study was to investigate the numerical relationships of FRNA-bacteriophage genotypes, adenovirus 41, and human adenoviruses (HADV) in Mexican surface water systems to assess sewage contamination. We studied the presence of HADV, HADV41 and FRNA bacteriophage genotypes in water samples and quantified by qPCR and RT-qPCR. Virus and water quality indicator variances, as analyzed by principal component analysis and partial least squared regression, followed along the major percentiles of water faecal enterococci. FRNA bacteriophages adequately deciphered viral and point source water contamination. The strongest correlation for HADV was with FRNA bacteriophage type II, in water samples higher than the 50(th) percentiles of faecal enterococci, thus indicating urban pollution. FRNA bacteriophage genotypes I and III virus indicator performances were assisted by their associations with electrical conductivity and faecal enterococci. In combination, our methods are useful for inferring water quality degradation caused by sewage contamination. The methods used have potential for determining source contamination in water and, specifically, the presence of enteric viruses where clean and contaminated water have mixed.
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spelling pubmed-52569212017-02-06 FRNA Bacteriophages as Viral Indicators of Faecal Contamination in Mexican Tropical Aquatic Systems Arredondo-Hernandez, Luis Jose Rene Diaz-Avalos, Carlos Lopez-Vidal, Yolanda Castillo-Rojas, Gonzalo Mazari-Hiriart, Marisa PLoS One Research Article A particular challenge to water safety in populous intertropical regions is the lack of reliable faecal indicators to detect microbiological contamination of water, while the numerical relationships of specific viral indicators remain largely unexplored. The aim of this study was to investigate the numerical relationships of FRNA-bacteriophage genotypes, adenovirus 41, and human adenoviruses (HADV) in Mexican surface water systems to assess sewage contamination. We studied the presence of HADV, HADV41 and FRNA bacteriophage genotypes in water samples and quantified by qPCR and RT-qPCR. Virus and water quality indicator variances, as analyzed by principal component analysis and partial least squared regression, followed along the major percentiles of water faecal enterococci. FRNA bacteriophages adequately deciphered viral and point source water contamination. The strongest correlation for HADV was with FRNA bacteriophage type II, in water samples higher than the 50(th) percentiles of faecal enterococci, thus indicating urban pollution. FRNA bacteriophage genotypes I and III virus indicator performances were assisted by their associations with electrical conductivity and faecal enterococci. In combination, our methods are useful for inferring water quality degradation caused by sewage contamination. The methods used have potential for determining source contamination in water and, specifically, the presence of enteric viruses where clean and contaminated water have mixed. Public Library of Science 2017-01-23 /pmc/articles/PMC5256921/ /pubmed/28114378 http://dx.doi.org/10.1371/journal.pone.0170399 Text en © 2017 Arredondo-Hernandez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Arredondo-Hernandez, Luis Jose Rene
Diaz-Avalos, Carlos
Lopez-Vidal, Yolanda
Castillo-Rojas, Gonzalo
Mazari-Hiriart, Marisa
FRNA Bacteriophages as Viral Indicators of Faecal Contamination in Mexican Tropical Aquatic Systems
title FRNA Bacteriophages as Viral Indicators of Faecal Contamination in Mexican Tropical Aquatic Systems
title_full FRNA Bacteriophages as Viral Indicators of Faecal Contamination in Mexican Tropical Aquatic Systems
title_fullStr FRNA Bacteriophages as Viral Indicators of Faecal Contamination in Mexican Tropical Aquatic Systems
title_full_unstemmed FRNA Bacteriophages as Viral Indicators of Faecal Contamination in Mexican Tropical Aquatic Systems
title_short FRNA Bacteriophages as Viral Indicators of Faecal Contamination in Mexican Tropical Aquatic Systems
title_sort frna bacteriophages as viral indicators of faecal contamination in mexican tropical aquatic systems
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5256921/
https://www.ncbi.nlm.nih.gov/pubmed/28114378
http://dx.doi.org/10.1371/journal.pone.0170399
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