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Extracellular Alkalinization as a Defense Response in Potato Cells

A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance and also for the early identification of disease during pre- or non-symptomatic phases. An increase in extracellular pH is known to be an early defense response in plants. In this study...

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Autores principales: Moroz, Natalia, Fritch, Karen R., Marcec, Matthew J., Tripathi, Diwaker, Smertenko, Andrei, Tanaka, Kiwamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5258701/
https://www.ncbi.nlm.nih.gov/pubmed/28174578
http://dx.doi.org/10.3389/fpls.2017.00032
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author Moroz, Natalia
Fritch, Karen R.
Marcec, Matthew J.
Tripathi, Diwaker
Smertenko, Andrei
Tanaka, Kiwamu
author_facet Moroz, Natalia
Fritch, Karen R.
Marcec, Matthew J.
Tripathi, Diwaker
Smertenko, Andrei
Tanaka, Kiwamu
author_sort Moroz, Natalia
collection PubMed
description A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance and also for the early identification of disease during pre- or non-symptomatic phases. An increase in extracellular pH is known to be an early defense response in plants. In this study, we demonstrate extracellular alkalinization as a defense response in potatoes. Using potato suspension cell cultures, we observed an alkalinization response against various pathogen- and plant-derived elicitors in a dose- and time-dependent manner. We also assessed the defense response against a variety of potato pathogens, such as protists (Phytophthora infestans and Spongospora subterranea) and fungi (Verticillium dahliae and Colletotrichum coccodes). Our results show that extracellular pH increases within 30 min in proportion to the number of pathogen spores added. Consistently with the alkalinization effect, the higher transcription level of several defense-related genes and production of reactive oxygen species was observed. Our results demonstrate that the alkalinization response is an effective marker to study early stages of defense response in potatoes.
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spelling pubmed-52587012017-02-07 Extracellular Alkalinization as a Defense Response in Potato Cells Moroz, Natalia Fritch, Karen R. Marcec, Matthew J. Tripathi, Diwaker Smertenko, Andrei Tanaka, Kiwamu Front Plant Sci Plant Science A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance and also for the early identification of disease during pre- or non-symptomatic phases. An increase in extracellular pH is known to be an early defense response in plants. In this study, we demonstrate extracellular alkalinization as a defense response in potatoes. Using potato suspension cell cultures, we observed an alkalinization response against various pathogen- and plant-derived elicitors in a dose- and time-dependent manner. We also assessed the defense response against a variety of potato pathogens, such as protists (Phytophthora infestans and Spongospora subterranea) and fungi (Verticillium dahliae and Colletotrichum coccodes). Our results show that extracellular pH increases within 30 min in proportion to the number of pathogen spores added. Consistently with the alkalinization effect, the higher transcription level of several defense-related genes and production of reactive oxygen species was observed. Our results demonstrate that the alkalinization response is an effective marker to study early stages of defense response in potatoes. Frontiers Media S.A. 2017-01-24 /pmc/articles/PMC5258701/ /pubmed/28174578 http://dx.doi.org/10.3389/fpls.2017.00032 Text en Copyright © 2017 Moroz, Fritch, Marcec, Tripathi, Smertenko and Tanaka. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Moroz, Natalia
Fritch, Karen R.
Marcec, Matthew J.
Tripathi, Diwaker
Smertenko, Andrei
Tanaka, Kiwamu
Extracellular Alkalinization as a Defense Response in Potato Cells
title Extracellular Alkalinization as a Defense Response in Potato Cells
title_full Extracellular Alkalinization as a Defense Response in Potato Cells
title_fullStr Extracellular Alkalinization as a Defense Response in Potato Cells
title_full_unstemmed Extracellular Alkalinization as a Defense Response in Potato Cells
title_short Extracellular Alkalinization as a Defense Response in Potato Cells
title_sort extracellular alkalinization as a defense response in potato cells
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5258701/
https://www.ncbi.nlm.nih.gov/pubmed/28174578
http://dx.doi.org/10.3389/fpls.2017.00032
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