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ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India

Melocanna baccifera (Roxb.) Kurz is an economically important bamboo of North-East India experiencing population depletion in its natural habitats. Genetic variation studies were conducted in 7 populations sampled from 5 districts of Manipur using ISSR molecular markers. The investigation was carrie...

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Autores principales: Nilkanta, Heikrujam, Amom, Thoungamba, Tikendra, Leimapokpam, Rahaman, Hamidur, Nongdam, Potshangbam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259607/
https://www.ncbi.nlm.nih.gov/pubmed/28168084
http://dx.doi.org/10.1155/2017/3757238
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author Nilkanta, Heikrujam
Amom, Thoungamba
Tikendra, Leimapokpam
Rahaman, Hamidur
Nongdam, Potshangbam
author_facet Nilkanta, Heikrujam
Amom, Thoungamba
Tikendra, Leimapokpam
Rahaman, Hamidur
Nongdam, Potshangbam
author_sort Nilkanta, Heikrujam
collection PubMed
description Melocanna baccifera (Roxb.) Kurz is an economically important bamboo of North-East India experiencing population depletion in its natural habitats. Genetic variation studies were conducted in 7 populations sampled from 5 districts of Manipur using ISSR molecular markers. The investigation was carried out as a primary step towards developing effective conservation strategies for the protection of bamboo germplasm. ISSR marker analysis showed significant level of genetic variation within the populations as revealed by moderately high average values of Nei's genetic diversity (H 0.1639), Shannon's diversity index (I 0.2563), percentage of polymorphic bands (PPB 59.18), total genetic variation (H(t) 0.1961), and genetic diversity within population (H(s) 0.1639). The study also divulged a high genetic variation at species level with Shannon's diversity index (I), Nei's genetic diversity (H), and percentage of polymorphic band (PPB%) recorded at 0.3218, 0.1939, and 88.37, respectively. Genetic differentiation among the populations (G(st)) was merely 19.42% leaving 80.58% of genetic variation exhibited within the populations. The low genetic diversity between populations was consistent with AMOVA. The low genetic differentiation among populations coupled with existence of significantly high genetic diversity at species level indicated the urgent necessity of preserving and protecting all the existing natural bamboo populations in the region.
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spelling pubmed-52596072017-02-06 ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India Nilkanta, Heikrujam Amom, Thoungamba Tikendra, Leimapokpam Rahaman, Hamidur Nongdam, Potshangbam Scientifica (Cairo) Research Article Melocanna baccifera (Roxb.) Kurz is an economically important bamboo of North-East India experiencing population depletion in its natural habitats. Genetic variation studies were conducted in 7 populations sampled from 5 districts of Manipur using ISSR molecular markers. The investigation was carried out as a primary step towards developing effective conservation strategies for the protection of bamboo germplasm. ISSR marker analysis showed significant level of genetic variation within the populations as revealed by moderately high average values of Nei's genetic diversity (H 0.1639), Shannon's diversity index (I 0.2563), percentage of polymorphic bands (PPB 59.18), total genetic variation (H(t) 0.1961), and genetic diversity within population (H(s) 0.1639). The study also divulged a high genetic variation at species level with Shannon's diversity index (I), Nei's genetic diversity (H), and percentage of polymorphic band (PPB%) recorded at 0.3218, 0.1939, and 88.37, respectively. Genetic differentiation among the populations (G(st)) was merely 19.42% leaving 80.58% of genetic variation exhibited within the populations. The low genetic diversity between populations was consistent with AMOVA. The low genetic differentiation among populations coupled with existence of significantly high genetic diversity at species level indicated the urgent necessity of preserving and protecting all the existing natural bamboo populations in the region. Hindawi Publishing Corporation 2017 2017-01-10 /pmc/articles/PMC5259607/ /pubmed/28168084 http://dx.doi.org/10.1155/2017/3757238 Text en Copyright © 2017 Heikrujam Nilkanta et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nilkanta, Heikrujam
Amom, Thoungamba
Tikendra, Leimapokpam
Rahaman, Hamidur
Nongdam, Potshangbam
ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India
title ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India
title_full ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India
title_fullStr ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India
title_full_unstemmed ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India
title_short ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India
title_sort issr marker based population genetic study of melocanna baccifera (roxb.) kurz: a commercially important bamboo of manipur, north-east india
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259607/
https://www.ncbi.nlm.nih.gov/pubmed/28168084
http://dx.doi.org/10.1155/2017/3757238
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