Assessment of the Fecal Microbiota in Beef Calves

BACKGROUND: There is increasing interest in the fecal microbiota, but study in calves has been limited. HYPOTHESIS/OBJECTIVES: To evaluate the fecal microbiota of beef calves and cows on different farms, and to preliminarily explore the impact of antimicrobial exposure. ANIMALS: A total of 172 anima...

Descripción completa

Detalles Bibliográficos
Autores principales: Weese, J.S., Jelinski, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259625/
https://www.ncbi.nlm.nih.gov/pubmed/27873352
http://dx.doi.org/10.1111/jvim.14611
_version_ 1782499239572537344
author Weese, J.S.
Jelinski, M.
author_facet Weese, J.S.
Jelinski, M.
author_sort Weese, J.S.
collection PubMed
description BACKGROUND: There is increasing interest in the fecal microbiota, but study in calves has been limited. HYPOTHESIS/OBJECTIVES: To evaluate the fecal microbiota of beef calves and cows on different farms, and to preliminarily explore the impact of antimicrobial exposure. ANIMALS: A total of 172 animals, 156 (91%) calves and 16 (9.3%) cows, were enrolled from 5 cow‐calf farms. METHODS: The fecal bacterial microbiota was assessed through sequencing of 16S rRNA gene (V4 region) amplicons. RESULTS: There were significant differences in the relative abundances of numerous phyla between calves on different farms. Farms could be separated into 2 groups: 1 (farms B and C) dominated by Firmicutes and 1 (farms A, D, and E) with predominance of Proteobacteria and Actinobacteria. Richness (median 2,974 versus 1,477, P = .008), diversity (51.4 versus 29.1, P = .0029), and evenness (0.73 versus 0.68, P = .006) were higher in cows. Over‐represented operational taxonomic units (OTUs) in cows tended to be from the classes Bacilli and Bacteroidia, whereas Clostridia and Actinobacteria were most prominently over‐represented in calves. There were differences in community membership (P = .028) and structure (P = .029) in calves that had a history of antimicrobial exposure compared those that did not. Eight (89%) over‐represented OTUs in the untreated group were Firmicutes (7 from the order Clostridiales), compared to only 3 (38%) (2 Clostridiales) in the untreated group. CONCLUSIONS AND CLINICAL IMPORTANCE: Interfarm variation should be investigated to determine the causes and potential implications for health and production. Antimicrobial exposure may have an impact on the fecal microbiota at individual and farm levels.
format Online
Article
Text
id pubmed-5259625
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-52596252017-01-30 Assessment of the Fecal Microbiota in Beef Calves Weese, J.S. Jelinski, M. J Vet Intern Med FOOD AND FIBER ANIMAL BACKGROUND: There is increasing interest in the fecal microbiota, but study in calves has been limited. HYPOTHESIS/OBJECTIVES: To evaluate the fecal microbiota of beef calves and cows on different farms, and to preliminarily explore the impact of antimicrobial exposure. ANIMALS: A total of 172 animals, 156 (91%) calves and 16 (9.3%) cows, were enrolled from 5 cow‐calf farms. METHODS: The fecal bacterial microbiota was assessed through sequencing of 16S rRNA gene (V4 region) amplicons. RESULTS: There were significant differences in the relative abundances of numerous phyla between calves on different farms. Farms could be separated into 2 groups: 1 (farms B and C) dominated by Firmicutes and 1 (farms A, D, and E) with predominance of Proteobacteria and Actinobacteria. Richness (median 2,974 versus 1,477, P = .008), diversity (51.4 versus 29.1, P = .0029), and evenness (0.73 versus 0.68, P = .006) were higher in cows. Over‐represented operational taxonomic units (OTUs) in cows tended to be from the classes Bacilli and Bacteroidia, whereas Clostridia and Actinobacteria were most prominently over‐represented in calves. There were differences in community membership (P = .028) and structure (P = .029) in calves that had a history of antimicrobial exposure compared those that did not. Eight (89%) over‐represented OTUs in the untreated group were Firmicutes (7 from the order Clostridiales), compared to only 3 (38%) (2 Clostridiales) in the untreated group. CONCLUSIONS AND CLINICAL IMPORTANCE: Interfarm variation should be investigated to determine the causes and potential implications for health and production. Antimicrobial exposure may have an impact on the fecal microbiota at individual and farm levels. John Wiley and Sons Inc. 2016-11-22 2017 /pmc/articles/PMC5259625/ /pubmed/27873352 http://dx.doi.org/10.1111/jvim.14611 Text en Copyright © 2016 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle FOOD AND FIBER ANIMAL
Weese, J.S.
Jelinski, M.
Assessment of the Fecal Microbiota in Beef Calves
title Assessment of the Fecal Microbiota in Beef Calves
title_full Assessment of the Fecal Microbiota in Beef Calves
title_fullStr Assessment of the Fecal Microbiota in Beef Calves
title_full_unstemmed Assessment of the Fecal Microbiota in Beef Calves
title_short Assessment of the Fecal Microbiota in Beef Calves
title_sort assessment of the fecal microbiota in beef calves
topic FOOD AND FIBER ANIMAL
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259625/
https://www.ncbi.nlm.nih.gov/pubmed/27873352
http://dx.doi.org/10.1111/jvim.14611
work_keys_str_mv AT weesejs assessmentofthefecalmicrobiotainbeefcalves
AT jelinskim assessmentofthefecalmicrobiotainbeefcalves