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A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol

In the present work, we demonstrate the fabrication of laccase biosensor to detect the catechol (CC) using laccase immobilized on graphene-cellulose microfibers (GR-CMF) composite modified screen printed carbon electrode (SPCE). The direct electrochemical behavior of laccase was investigated using l...

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Autores principales: Palanisamy, Selvakumar, Ramaraj, Sayee Kannan, Chen, Shen-Ming, Yang, Thomas C. K., Yi-Fan, Pan, Chen, Tse-Wei, Velusamy, Vijayalakshmi, Selvam, Sonadevi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259700/
https://www.ncbi.nlm.nih.gov/pubmed/28117357
http://dx.doi.org/10.1038/srep41214
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author Palanisamy, Selvakumar
Ramaraj, Sayee Kannan
Chen, Shen-Ming
Yang, Thomas C. K.
Yi-Fan, Pan
Chen, Tse-Wei
Velusamy, Vijayalakshmi
Selvam, Sonadevi
author_facet Palanisamy, Selvakumar
Ramaraj, Sayee Kannan
Chen, Shen-Ming
Yang, Thomas C. K.
Yi-Fan, Pan
Chen, Tse-Wei
Velusamy, Vijayalakshmi
Selvam, Sonadevi
author_sort Palanisamy, Selvakumar
collection PubMed
description In the present work, we demonstrate the fabrication of laccase biosensor to detect the catechol (CC) using laccase immobilized on graphene-cellulose microfibers (GR-CMF) composite modified screen printed carbon electrode (SPCE). The direct electrochemical behavior of laccase was investigated using laccase immobilized different modified SPCEs, such as GR/SPCE, CMF/SPCE and GR-CMF/SPCE. Compared with laccase immobilized GR and CMF modified SPCEs, a well-defined redox couple of Cu(I)/Cu(II) for laccase was observed at laccase immobilized GR-CMF composite modified SPCE. Cyclic voltammetry results show that the as-prepared biosensor has 7 folds higher catalytic activity with lower oxidation potential towards CC than SPCE modified with GR-CMF composite. Under optimized conditions, amperometric i-t method was used for the quantification of CC, and the amperometric response of the biosensor was linear over the concertation of CC ranging from 0.2 to 209.7 μM. The sensitivity, response time and the detection limit of the biosensor for CC is 0.932 μMμA(−1) cm(−2), 2 s and 0.085 μM, respectively. The biosensor has high selectivity towards CC in the presence of potentially active biomolecules and phenolic compounds. The biosensor also accessed for the detection of CC in different water samples and shows good practicality with an appropriate repea.
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spelling pubmed-52597002017-01-24 A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol Palanisamy, Selvakumar Ramaraj, Sayee Kannan Chen, Shen-Ming Yang, Thomas C. K. Yi-Fan, Pan Chen, Tse-Wei Velusamy, Vijayalakshmi Selvam, Sonadevi Sci Rep Article In the present work, we demonstrate the fabrication of laccase biosensor to detect the catechol (CC) using laccase immobilized on graphene-cellulose microfibers (GR-CMF) composite modified screen printed carbon electrode (SPCE). The direct electrochemical behavior of laccase was investigated using laccase immobilized different modified SPCEs, such as GR/SPCE, CMF/SPCE and GR-CMF/SPCE. Compared with laccase immobilized GR and CMF modified SPCEs, a well-defined redox couple of Cu(I)/Cu(II) for laccase was observed at laccase immobilized GR-CMF composite modified SPCE. Cyclic voltammetry results show that the as-prepared biosensor has 7 folds higher catalytic activity with lower oxidation potential towards CC than SPCE modified with GR-CMF composite. Under optimized conditions, amperometric i-t method was used for the quantification of CC, and the amperometric response of the biosensor was linear over the concertation of CC ranging from 0.2 to 209.7 μM. The sensitivity, response time and the detection limit of the biosensor for CC is 0.932 μMμA(−1) cm(−2), 2 s and 0.085 μM, respectively. The biosensor has high selectivity towards CC in the presence of potentially active biomolecules and phenolic compounds. The biosensor also accessed for the detection of CC in different water samples and shows good practicality with an appropriate repea. Nature Publishing Group 2017-01-24 /pmc/articles/PMC5259700/ /pubmed/28117357 http://dx.doi.org/10.1038/srep41214 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Palanisamy, Selvakumar
Ramaraj, Sayee Kannan
Chen, Shen-Ming
Yang, Thomas C. K.
Yi-Fan, Pan
Chen, Tse-Wei
Velusamy, Vijayalakshmi
Selvam, Sonadevi
A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol
title A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol
title_full A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol
title_fullStr A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol
title_full_unstemmed A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol
title_short A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol
title_sort novel laccase biosensor based on laccase immobilized graphene-cellulose microfiber composite modified screen-printed carbon electrode for sensitive determination of catechol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259700/
https://www.ncbi.nlm.nih.gov/pubmed/28117357
http://dx.doi.org/10.1038/srep41214
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