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Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains

Planktothrix is a dominant cyanobacterial genus forming toxic blooms in temperate freshwater ecosystems. We sequenced the genome of planktic and non planktic Planktothrix strains to better represent this genus diversity and life style at the genomic level. Benthic and biphasic strains are rooting th...

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Autores principales: Pancrace, Claire, Barny, Marie-Anne, Ueoka, Reiko, Calteau, Alexandra, Scalvenzi, Thibault, Pédron, Jacques, Barbe, Valérie, Piel, Joern, Humbert, Jean-François, Gugger, Muriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259702/
https://www.ncbi.nlm.nih.gov/pubmed/28117406
http://dx.doi.org/10.1038/srep41181
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author Pancrace, Claire
Barny, Marie-Anne
Ueoka, Reiko
Calteau, Alexandra
Scalvenzi, Thibault
Pédron, Jacques
Barbe, Valérie
Piel, Joern
Humbert, Jean-François
Gugger, Muriel
author_facet Pancrace, Claire
Barny, Marie-Anne
Ueoka, Reiko
Calteau, Alexandra
Scalvenzi, Thibault
Pédron, Jacques
Barbe, Valérie
Piel, Joern
Humbert, Jean-François
Gugger, Muriel
author_sort Pancrace, Claire
collection PubMed
description Planktothrix is a dominant cyanobacterial genus forming toxic blooms in temperate freshwater ecosystems. We sequenced the genome of planktic and non planktic Planktothrix strains to better represent this genus diversity and life style at the genomic level. Benthic and biphasic strains are rooting the Planktothrix phylogenetic tree and widely expand the pangenome of this genus. We further investigated in silico the genetic potential dedicated to gas vesicles production, nitrogen fixation as well as natural product synthesis and conducted complementary experimental tests by cell culture, microscopy and mass spectrometry. Significant differences for the investigated features could be evidenced between strains of different life styles. The benthic Planktothrix strains showed unexpected characteristics such as buoyancy, nitrogen fixation capacity and unique natural product features. In comparison with Microcystis, another dominant toxic bloom-forming genus in freshwater ecosystem, different evolutionary strategies were highlighted notably as Planktothrix exhibits an overall greater genetic diversity but a smaller genomic plasticity than Microcystis. Our results are shedding light on Planktothrix evolution, phylogeny and physiology in the frame of their diverse life styles.
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spelling pubmed-52597022017-01-24 Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains Pancrace, Claire Barny, Marie-Anne Ueoka, Reiko Calteau, Alexandra Scalvenzi, Thibault Pédron, Jacques Barbe, Valérie Piel, Joern Humbert, Jean-François Gugger, Muriel Sci Rep Article Planktothrix is a dominant cyanobacterial genus forming toxic blooms in temperate freshwater ecosystems. We sequenced the genome of planktic and non planktic Planktothrix strains to better represent this genus diversity and life style at the genomic level. Benthic and biphasic strains are rooting the Planktothrix phylogenetic tree and widely expand the pangenome of this genus. We further investigated in silico the genetic potential dedicated to gas vesicles production, nitrogen fixation as well as natural product synthesis and conducted complementary experimental tests by cell culture, microscopy and mass spectrometry. Significant differences for the investigated features could be evidenced between strains of different life styles. The benthic Planktothrix strains showed unexpected characteristics such as buoyancy, nitrogen fixation capacity and unique natural product features. In comparison with Microcystis, another dominant toxic bloom-forming genus in freshwater ecosystem, different evolutionary strategies were highlighted notably as Planktothrix exhibits an overall greater genetic diversity but a smaller genomic plasticity than Microcystis. Our results are shedding light on Planktothrix evolution, phylogeny and physiology in the frame of their diverse life styles. Nature Publishing Group 2017-01-24 /pmc/articles/PMC5259702/ /pubmed/28117406 http://dx.doi.org/10.1038/srep41181 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Pancrace, Claire
Barny, Marie-Anne
Ueoka, Reiko
Calteau, Alexandra
Scalvenzi, Thibault
Pédron, Jacques
Barbe, Valérie
Piel, Joern
Humbert, Jean-François
Gugger, Muriel
Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains
title Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains
title_full Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains
title_fullStr Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains
title_full_unstemmed Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains
title_short Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains
title_sort insights into the planktothrix genus: genomic and metabolic comparison of benthic and planktic strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259702/
https://www.ncbi.nlm.nih.gov/pubmed/28117406
http://dx.doi.org/10.1038/srep41181
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