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Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury

Ependyma have been proposed as adult neural stem cells that provide the majority of newly proliferated scar-forming astrocytes that protect tissue and function after spinal cord injury (SCI). This proposal was based on small, midline stab SCI. Here, we tested the generality of this proposal by using...

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Autores principales: Ren, Yilong, Ao, Yan, O’Shea, Timothy M., Burda, Joshua E., Bernstein, Alexander M., Brumm, Andrew J., Muthusamy, Nagendran, Ghashghaei, H. Troy, Carmichael, S. Thomas, Cheng, Liming, Sofroniew, Michael V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259707/
https://www.ncbi.nlm.nih.gov/pubmed/28117356
http://dx.doi.org/10.1038/srep41122
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author Ren, Yilong
Ao, Yan
O’Shea, Timothy M.
Burda, Joshua E.
Bernstein, Alexander M.
Brumm, Andrew J.
Muthusamy, Nagendran
Ghashghaei, H. Troy
Carmichael, S. Thomas
Cheng, Liming
Sofroniew, Michael V.
author_facet Ren, Yilong
Ao, Yan
O’Shea, Timothy M.
Burda, Joshua E.
Bernstein, Alexander M.
Brumm, Andrew J.
Muthusamy, Nagendran
Ghashghaei, H. Troy
Carmichael, S. Thomas
Cheng, Liming
Sofroniew, Michael V.
author_sort Ren, Yilong
collection PubMed
description Ependyma have been proposed as adult neural stem cells that provide the majority of newly proliferated scar-forming astrocytes that protect tissue and function after spinal cord injury (SCI). This proposal was based on small, midline stab SCI. Here, we tested the generality of this proposal by using a genetic knock-in cell fate mapping strategy in different murine SCI models. After large crush injuries across the entire spinal cord, ependyma-derived progeny remained local, did not migrate and contributed few cells of any kind and less than 2%, if any, of the total newly proliferated and molecularly confirmed scar-forming astrocytes. Stab injuries that were near to but did not directly damage ependyma, contained no ependyma-derived cells. Our findings show that ependymal contribution of progeny after SCI is minimal, local and dependent on direct ependymal injury, indicating that ependyma are not a major source of endogenous neural stem cells or neuroprotective astrocytes after SCI.
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spelling pubmed-52597072017-01-24 Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury Ren, Yilong Ao, Yan O’Shea, Timothy M. Burda, Joshua E. Bernstein, Alexander M. Brumm, Andrew J. Muthusamy, Nagendran Ghashghaei, H. Troy Carmichael, S. Thomas Cheng, Liming Sofroniew, Michael V. Sci Rep Article Ependyma have been proposed as adult neural stem cells that provide the majority of newly proliferated scar-forming astrocytes that protect tissue and function after spinal cord injury (SCI). This proposal was based on small, midline stab SCI. Here, we tested the generality of this proposal by using a genetic knock-in cell fate mapping strategy in different murine SCI models. After large crush injuries across the entire spinal cord, ependyma-derived progeny remained local, did not migrate and contributed few cells of any kind and less than 2%, if any, of the total newly proliferated and molecularly confirmed scar-forming astrocytes. Stab injuries that were near to but did not directly damage ependyma, contained no ependyma-derived cells. Our findings show that ependymal contribution of progeny after SCI is minimal, local and dependent on direct ependymal injury, indicating that ependyma are not a major source of endogenous neural stem cells or neuroprotective astrocytes after SCI. Nature Publishing Group 2017-01-24 /pmc/articles/PMC5259707/ /pubmed/28117356 http://dx.doi.org/10.1038/srep41122 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ren, Yilong
Ao, Yan
O’Shea, Timothy M.
Burda, Joshua E.
Bernstein, Alexander M.
Brumm, Andrew J.
Muthusamy, Nagendran
Ghashghaei, H. Troy
Carmichael, S. Thomas
Cheng, Liming
Sofroniew, Michael V.
Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury
title Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury
title_full Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury
title_fullStr Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury
title_full_unstemmed Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury
title_short Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury
title_sort ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259707/
https://www.ncbi.nlm.nih.gov/pubmed/28117356
http://dx.doi.org/10.1038/srep41122
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