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MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae
Endocytosis is a crucial cellular process in eukaryotic cells which involves clathrin and/or adaptor proteins, lipid kinases, phosphatases and the actin cytoskeleton. Verprolin proteins, such as Vrp1 in Saccharomyces cerevisiae, are conserved family proteins that regulate actin binding and endocytos...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259722/ https://www.ncbi.nlm.nih.gov/pubmed/28117435 http://dx.doi.org/10.1038/srep41148 |
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author | Huang, Lin Zhang, Shengpei Yin, Ziyi Liu, Muxing Li, Bing Zhang, Haifeng Zheng, Xiaobo Wang, Ping Zhang, Zhengguang |
author_facet | Huang, Lin Zhang, Shengpei Yin, Ziyi Liu, Muxing Li, Bing Zhang, Haifeng Zheng, Xiaobo Wang, Ping Zhang, Zhengguang |
author_sort | Huang, Lin |
collection | PubMed |
description | Endocytosis is a crucial cellular process in eukaryotic cells which involves clathrin and/or adaptor proteins, lipid kinases, phosphatases and the actin cytoskeleton. Verprolin proteins, such as Vrp1 in Saccharomyces cerevisiae, are conserved family proteins that regulate actin binding and endocytosis. Here, we identified and characterized MoVrp1 as the yeast Vrp1 homolog in Magnaporthe oryzae. Deletion of the MoVRP1 gene resulted in defects in vegetative growth, asexual development, and infection of the host plant. The ∆Movrp1 mutants also exhibited decreased extracellular peroxidase and laccase activities and showed defects in colony pigmentation, hyphal surface hydrophobicity, cell wall integrity, autophagy, endocytosis, and secretion of avirulent effector. Our studies provided new evidences that MoVrp1 involved in actin cytoskeleton is important for growth, morphogenesis, cellular trafficking, and fungal pathogenesis. |
format | Online Article Text |
id | pubmed-5259722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52597222017-01-24 MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae Huang, Lin Zhang, Shengpei Yin, Ziyi Liu, Muxing Li, Bing Zhang, Haifeng Zheng, Xiaobo Wang, Ping Zhang, Zhengguang Sci Rep Article Endocytosis is a crucial cellular process in eukaryotic cells which involves clathrin and/or adaptor proteins, lipid kinases, phosphatases and the actin cytoskeleton. Verprolin proteins, such as Vrp1 in Saccharomyces cerevisiae, are conserved family proteins that regulate actin binding and endocytosis. Here, we identified and characterized MoVrp1 as the yeast Vrp1 homolog in Magnaporthe oryzae. Deletion of the MoVRP1 gene resulted in defects in vegetative growth, asexual development, and infection of the host plant. The ∆Movrp1 mutants also exhibited decreased extracellular peroxidase and laccase activities and showed defects in colony pigmentation, hyphal surface hydrophobicity, cell wall integrity, autophagy, endocytosis, and secretion of avirulent effector. Our studies provided new evidences that MoVrp1 involved in actin cytoskeleton is important for growth, morphogenesis, cellular trafficking, and fungal pathogenesis. Nature Publishing Group 2017-01-24 /pmc/articles/PMC5259722/ /pubmed/28117435 http://dx.doi.org/10.1038/srep41148 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Huang, Lin Zhang, Shengpei Yin, Ziyi Liu, Muxing Li, Bing Zhang, Haifeng Zheng, Xiaobo Wang, Ping Zhang, Zhengguang MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae |
title | MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae |
title_full | MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae |
title_fullStr | MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae |
title_full_unstemmed | MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae |
title_short | MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae |
title_sort | movrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus magnaporthe oryzae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259722/ https://www.ncbi.nlm.nih.gov/pubmed/28117435 http://dx.doi.org/10.1038/srep41148 |
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