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Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice

BACKGROUND: Young female patients who receive chemotherapy frequently face premature ovarian failure (POF). The therapeutic potential of stem cells in these patients has been explored in stem cells derived from different sources. However, many of these types of stem cells are either difficult to obt...

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Autores principales: Wang, Zhen, Wang, Yueling, Yang, Ting, Li, Jing, Yang, Xinyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259841/
https://www.ncbi.nlm.nih.gov/pubmed/28114977
http://dx.doi.org/10.1186/s13287-016-0458-1
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author Wang, Zhen
Wang, Yueling
Yang, Ting
Li, Jing
Yang, Xinyuan
author_facet Wang, Zhen
Wang, Yueling
Yang, Ting
Li, Jing
Yang, Xinyuan
author_sort Wang, Zhen
collection PubMed
description BACKGROUND: Young female patients who receive chemotherapy frequently face premature ovarian failure (POF). The therapeutic potential of stem cells in these patients has been explored in stem cells derived from different sources. However, many of these types of stem cells are either difficult to obtain or obtaining them involves invasive procedures. Here, we show that menstrual-derived stem cells (MenSCs) are easy to access and exhibit mesenchymal stem cell-like properties. MenSCs are therefore a novel source of stem cells that can be used for tissue repair. The aim of this study was to explore the reparative capacity and the mechanism underlying the activities of MenSCs. METHODS: POF mouse models were established by 7 consecutive days of intraperitoneal injection of cisplatin, and then MenSCs or MenSC-derived conditioned media (CM) were infused via the tail vein. The ovaries were excised after either 7 or 21 days of treatment and the follicles were counted and categorized. Apoptosis of granulosa cells was observed by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling staining. Ovarian function was evaluated by monitoring serum sex hormone levels. Furthermore, MenSC tracking, Q-PCR, and small interfering RNA transfection were used to reveal the inner mechanism of repair. RESULTS: MenSC transplantation could improve the ovarian microenvironment by reducing apoptosis in granulosa cells and the fibrosis of ovarian interstitium, which contributes to increase the follicular numbers and return sex hormone levels to normal values. Meanwhile, the transplanted MenSCs directively migrate to ovarian interstitium to play a role in repair rather than differentiate to oocytes directly. Additionally, MenSCs and CM derived from these cells exerted protective effects on damaged ovaries partially by secreting FGF2. CONCLUSION: MenSCs repair ovarian injury, improve ovarian function, and stimulate regeneration, suggesting that transplantation of MenSCs may provide an effective and novel method for treating POF.
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spelling pubmed-52598412017-01-26 Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice Wang, Zhen Wang, Yueling Yang, Ting Li, Jing Yang, Xinyuan Stem Cell Res Ther Research BACKGROUND: Young female patients who receive chemotherapy frequently face premature ovarian failure (POF). The therapeutic potential of stem cells in these patients has been explored in stem cells derived from different sources. However, many of these types of stem cells are either difficult to obtain or obtaining them involves invasive procedures. Here, we show that menstrual-derived stem cells (MenSCs) are easy to access and exhibit mesenchymal stem cell-like properties. MenSCs are therefore a novel source of stem cells that can be used for tissue repair. The aim of this study was to explore the reparative capacity and the mechanism underlying the activities of MenSCs. METHODS: POF mouse models were established by 7 consecutive days of intraperitoneal injection of cisplatin, and then MenSCs or MenSC-derived conditioned media (CM) were infused via the tail vein. The ovaries were excised after either 7 or 21 days of treatment and the follicles were counted and categorized. Apoptosis of granulosa cells was observed by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling staining. Ovarian function was evaluated by monitoring serum sex hormone levels. Furthermore, MenSC tracking, Q-PCR, and small interfering RNA transfection were used to reveal the inner mechanism of repair. RESULTS: MenSC transplantation could improve the ovarian microenvironment by reducing apoptosis in granulosa cells and the fibrosis of ovarian interstitium, which contributes to increase the follicular numbers and return sex hormone levels to normal values. Meanwhile, the transplanted MenSCs directively migrate to ovarian interstitium to play a role in repair rather than differentiate to oocytes directly. Additionally, MenSCs and CM derived from these cells exerted protective effects on damaged ovaries partially by secreting FGF2. CONCLUSION: MenSCs repair ovarian injury, improve ovarian function, and stimulate regeneration, suggesting that transplantation of MenSCs may provide an effective and novel method for treating POF. BioMed Central 2017-01-23 /pmc/articles/PMC5259841/ /pubmed/28114977 http://dx.doi.org/10.1186/s13287-016-0458-1 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wang, Zhen
Wang, Yueling
Yang, Ting
Li, Jing
Yang, Xinyuan
Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
title Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
title_full Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
title_fullStr Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
title_full_unstemmed Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
title_short Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
title_sort study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259841/
https://www.ncbi.nlm.nih.gov/pubmed/28114977
http://dx.doi.org/10.1186/s13287-016-0458-1
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