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Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast
Octacalcium phosphate (OCP) and hydroxyapatite (HAp) coatings were developed to control the degradation speed and to improve the biocompatibility of biodegradable magnesium alloys. Osteoblast MG-63 was cultured directly on OCP- and HAp-coated Mg-3Al-1Zn (wt%, AZ31) alloy (OCP- and HAp-AZ31) to evalu...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259964/ https://www.ncbi.nlm.nih.gov/pubmed/28179963 http://dx.doi.org/10.1080/14686996.2016.1266238 |
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author | Hiromoto, Sachiko Yamazaki, Tomohiko |
author_facet | Hiromoto, Sachiko Yamazaki, Tomohiko |
author_sort | Hiromoto, Sachiko |
collection | PubMed |
description | Octacalcium phosphate (OCP) and hydroxyapatite (HAp) coatings were developed to control the degradation speed and to improve the biocompatibility of biodegradable magnesium alloys. Osteoblast MG-63 was cultured directly on OCP- and HAp-coated Mg-3Al-1Zn (wt%, AZ31) alloy (OCP- and HAp-AZ31) to evaluate cell compatibility. Cell proliferation was remarkably improved with OCP and HAp coatings which reduced the corrosion and prevented the H(2)O(2) generation on Mg alloy substrate. OCP-AZ31 showed sparse distribution of living cell colonies and dead cells. HAp-AZ31 showed dense and homogeneous distribution of living cells, with dead cells localized over and around corrosion pits, some of which were formed underneath the coating. These results demonstrated that cells were dead due to changes in the local environment, and it is necessary to evaluate the local biocompatibility of magnesium alloys. Cell density on HAp-AZ31 was higher than that on OCP-AZ31 although there was not a significant difference in the amount of Mg ions released in medium between OCP- and HAp-AZ31. The outer layer of OCP and HAp coatings consisted of plate-like crystal with a thickness of around 0.1 μm and rod-like crystals with a diameter of around 0.1 μm, respectively, which grew from a continuous inner layer. Osteoblasts formed focal contacts on the tips of plate-like OCP and rod-like HAp crystals, with heights of 2–5 μm. The spacing between OCP tips of 0.8–1.1 μm was wider than that between HAp tips of 0.2–0.3 μm. These results demonstrated that cell proliferation depended on the micromorphology of the coatings which governed spacing of focal contacts. Consequently, HAp coating is suitable for improving cell compatibility and bone-forming ability of the Mg alloy. |
format | Online Article Text |
id | pubmed-5259964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-52599642017-02-08 Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast Hiromoto, Sachiko Yamazaki, Tomohiko Sci Technol Adv Mater Bio-Inspired and Biomedical Materials Octacalcium phosphate (OCP) and hydroxyapatite (HAp) coatings were developed to control the degradation speed and to improve the biocompatibility of biodegradable magnesium alloys. Osteoblast MG-63 was cultured directly on OCP- and HAp-coated Mg-3Al-1Zn (wt%, AZ31) alloy (OCP- and HAp-AZ31) to evaluate cell compatibility. Cell proliferation was remarkably improved with OCP and HAp coatings which reduced the corrosion and prevented the H(2)O(2) generation on Mg alloy substrate. OCP-AZ31 showed sparse distribution of living cell colonies and dead cells. HAp-AZ31 showed dense and homogeneous distribution of living cells, with dead cells localized over and around corrosion pits, some of which were formed underneath the coating. These results demonstrated that cells were dead due to changes in the local environment, and it is necessary to evaluate the local biocompatibility of magnesium alloys. Cell density on HAp-AZ31 was higher than that on OCP-AZ31 although there was not a significant difference in the amount of Mg ions released in medium between OCP- and HAp-AZ31. The outer layer of OCP and HAp coatings consisted of plate-like crystal with a thickness of around 0.1 μm and rod-like crystals with a diameter of around 0.1 μm, respectively, which grew from a continuous inner layer. Osteoblasts formed focal contacts on the tips of plate-like OCP and rod-like HAp crystals, with heights of 2–5 μm. The spacing between OCP tips of 0.8–1.1 μm was wider than that between HAp tips of 0.2–0.3 μm. These results demonstrated that cell proliferation depended on the micromorphology of the coatings which governed spacing of focal contacts. Consequently, HAp coating is suitable for improving cell compatibility and bone-forming ability of the Mg alloy. Taylor & Francis 2017-01-23 /pmc/articles/PMC5259964/ /pubmed/28179963 http://dx.doi.org/10.1080/14686996.2016.1266238 Text en © 2017 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Bio-Inspired and Biomedical Materials Hiromoto, Sachiko Yamazaki, Tomohiko Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast |
title | Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast |
title_full | Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast |
title_fullStr | Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast |
title_full_unstemmed | Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast |
title_short | Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast |
title_sort | micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast |
topic | Bio-Inspired and Biomedical Materials |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259964/ https://www.ncbi.nlm.nih.gov/pubmed/28179963 http://dx.doi.org/10.1080/14686996.2016.1266238 |
work_keys_str_mv | AT hiromotosachiko micromorphologicaleffectofcalciumphosphatecoatingoncompatibilityofmagnesiumalloywithosteoblast AT yamazakitomohiko micromorphologicaleffectofcalciumphosphatecoatingoncompatibilityofmagnesiumalloywithosteoblast |