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Sox17 drives functional engraftment of endothelium converted from non-vascular cells
Transplanting vascular endothelial cells (ECs) to support metabolism and express regenerative paracrine factors is a strategy to treat vasculopathies and to promote tissue regeneration. However, transplantation strategies have been challenging to develop, because ECs are difficult to culture and lit...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5260855/ https://www.ncbi.nlm.nih.gov/pubmed/28091527 http://dx.doi.org/10.1038/ncomms13963 |
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author | Schachterle, William Badwe, Chaitanya R. Palikuqi, Brisa Kunar, Balvir Ginsberg, Michael Lis, Raphael Yokoyama, Masataka Elemento, Olivier Scandura, Joseph M. Rafii, Shahin |
author_facet | Schachterle, William Badwe, Chaitanya R. Palikuqi, Brisa Kunar, Balvir Ginsberg, Michael Lis, Raphael Yokoyama, Masataka Elemento, Olivier Scandura, Joseph M. Rafii, Shahin |
author_sort | Schachterle, William |
collection | PubMed |
description | Transplanting vascular endothelial cells (ECs) to support metabolism and express regenerative paracrine factors is a strategy to treat vasculopathies and to promote tissue regeneration. However, transplantation strategies have been challenging to develop, because ECs are difficult to culture and little is known about how to direct them to stably integrate into vasculature. Here we show that only amniotic cells could convert to cells that maintain EC gene expression. Even so, these converted cells perform sub-optimally in transplantation studies. Constitutive Akt signalling increases expression of EC morphogenesis genes, including Sox17, shifts the genomic targeting of Fli1 to favour nearby Sox consensus sites and enhances the vascular function of converted cells. Enforced expression of Sox17 increases expression of morphogenesis genes and promotes integration of transplanted converted cells into injured vessels. Thus, Ets transcription factors specify non-vascular, amniotic cells to EC-like cells, whereas Sox17 expression is required to confer EC function. |
format | Online Article Text |
id | pubmed-5260855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52608552017-01-25 Sox17 drives functional engraftment of endothelium converted from non-vascular cells Schachterle, William Badwe, Chaitanya R. Palikuqi, Brisa Kunar, Balvir Ginsberg, Michael Lis, Raphael Yokoyama, Masataka Elemento, Olivier Scandura, Joseph M. Rafii, Shahin Nat Commun Article Transplanting vascular endothelial cells (ECs) to support metabolism and express regenerative paracrine factors is a strategy to treat vasculopathies and to promote tissue regeneration. However, transplantation strategies have been challenging to develop, because ECs are difficult to culture and little is known about how to direct them to stably integrate into vasculature. Here we show that only amniotic cells could convert to cells that maintain EC gene expression. Even so, these converted cells perform sub-optimally in transplantation studies. Constitutive Akt signalling increases expression of EC morphogenesis genes, including Sox17, shifts the genomic targeting of Fli1 to favour nearby Sox consensus sites and enhances the vascular function of converted cells. Enforced expression of Sox17 increases expression of morphogenesis genes and promotes integration of transplanted converted cells into injured vessels. Thus, Ets transcription factors specify non-vascular, amniotic cells to EC-like cells, whereas Sox17 expression is required to confer EC function. Nature Publishing Group 2017-01-16 /pmc/articles/PMC5260855/ /pubmed/28091527 http://dx.doi.org/10.1038/ncomms13963 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Schachterle, William Badwe, Chaitanya R. Palikuqi, Brisa Kunar, Balvir Ginsberg, Michael Lis, Raphael Yokoyama, Masataka Elemento, Olivier Scandura, Joseph M. Rafii, Shahin Sox17 drives functional engraftment of endothelium converted from non-vascular cells |
title | Sox17 drives functional engraftment of endothelium converted from non-vascular cells |
title_full | Sox17 drives functional engraftment of endothelium converted from non-vascular cells |
title_fullStr | Sox17 drives functional engraftment of endothelium converted from non-vascular cells |
title_full_unstemmed | Sox17 drives functional engraftment of endothelium converted from non-vascular cells |
title_short | Sox17 drives functional engraftment of endothelium converted from non-vascular cells |
title_sort | sox17 drives functional engraftment of endothelium converted from non-vascular cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5260855/ https://www.ncbi.nlm.nih.gov/pubmed/28091527 http://dx.doi.org/10.1038/ncomms13963 |
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