Cargando…

Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool

CRISPR/Cas9 screening has proven to be a versatile tool for genomics research. Based on unexpected results from a genome-wide screen, we developed a CRISPR/Cas9-mediated approach to mutagenesis, exploiting the allelic diversity generated by error-prone non-homologous end-joining (NHEJ) to identify n...

Descripción completa

Detalles Bibliográficos
Autores principales:	Donovan, Katherine F., Hegde, Mudra, Sullender, Meagan, Vaimberg, Emma W., Johannessen, Cory M., Root, David E., Doench, John G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5261743/ https://www.ncbi.nlm.nih.gov/pubmed/28118392 http://dx.doi.org/10.1371/journal.pone.0170445

Ejemplares similares

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
por: Sanson, Kendall R., et al.
Publicado: (2018)

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
por: Doench, John G., et al.
Publicado: (2016)

Orthologous CRISPR-Cas9 enzymes for Combinatorial Genetic Screens
por: Najm, Fadi J, et al.
Publicado: (2017)

CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
por: Waaijers, Selma, et al.
Publicado: (2013)

CRISPR-Cas9-Mediated Mutagenesis: Mind the Gap?
por: Teboul, Lydia, et al.
Publicado: (2018)

CRISPR/Cas9-mediated targeted mutagenesis in grape
por: Nakajima, Ikuko, et al.
Publicado: (2017)

CRISPR-Cas9-Mediated Mutagenesis of the Rubisco Small Subunit Family in Nicotiana tabacum
por: Donovan, Sophie, et al.
Publicado: (2020)

Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
por: Hegde, Mudra, et al.
Publicado: (2018)

Targeted mutagenesis in soybean using the CRISPR-Cas9 system
por: Sun, Xianjun, et al.
Publicado: (2015)

Targeted mutagenesis in chicken using CRISPR/Cas9 system
por: Oishi, Isao, et al.
Publicado: (2016)

CRISPR/Cas9 Mediates Efficient Conditional Mutagenesis in Drosophila
por: Xue, Zhaoyu, et al.
Publicado: (2014)

CRISPR-Cas9 enables conditional mutagenesis of challenging loci
por: Schick, Joel A., et al.
Publicado: (2016)

Targeted mutagenesis in wheat microspores using CRISPR/Cas9
por: Bhowmik, Pankaj, et al.
Publicado: (2018)

CRISPR/Cas9-Mediated Mutagenesis of RCO in Cardamine hirsuta
por: Alvim Kamei, Claire Lessa, et al.
Publicado: (2020)

CRISPR/Cas9 Targeted Mutagenesis for Functional Genetics in Maize
por: Hunter, Charles T.
Publicado: (2021)

Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System
por: Bassett, Andrew R., et al.
Publicado: (2013)

Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia
por: Zhang, Bin, et al.
Publicado: (2016)

Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System
por: Bassett, Andrew R., et al.
Publicado: (2014)

CRISPR/Cas9-mediated mutagenesis of homologous genes in Chinese kale
por: Sun, Bo, et al.
Publicado: (2018)

Efficiency Optimization of CRISPR/Cas9-Mediated Targeted Mutagenesis in Grape
por: Ren, Fengrui, et al.
Publicado: (2019)

Efficient targeted mutagenesis in allotetraploid sweet basil by CRISPR/Cas9
por: Navet, Natasha, et al.
Publicado: (2020)

An Agrobacterium‐delivered CRISPR/Cas9 system for targeted mutagenesis in sorghum
por: Char, Si Nian, et al.
Publicado: (2019)

Efficient CRISPR/Cas9 mutagenesis for neurobehavioral screening in adult zebrafish
por: Klatt Shaw, Dana, et al.
Publicado: (2021)

CRISPR/Cas9 establishment-mediated targeted mutagenesis in Macrobrachium nipponense
por: Qiao, Hui, et al.
Publicado: (2023)

Creation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technology
por: Han, Jianan, et al.
Publicado: (2019)

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
por: Lee, Keunsub, et al.
Publicado: (2018)

CRISPR/Cas-Mediated Targeted Mutagenesis in Daphnia magna
por: Nakanishi, Takashi, et al.
Publicado: (2014)

Targeted mutagenesis in rice using CRISPR-Cas system
por: Miao, Jin, et al.
Publicado: (2013)

Targeted mutagenesis using CRISPR/Cas system in medaka
por: Ansai, Satoshi, et al.
Publicado: (2014)

CRISPR–Cas-mediated transcriptional control and epi-mutagenesis
por: Gardiner, Jason, et al.
Publicado: (2022)

CRISPR-Cas9 Technology for the Creation of Biological Avatars Capable of Modeling and Treating Pathologies: From Discovery to the Latest Improvements
por: Nasrallah, Ali, et al.
Publicado: (2022)

Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9
por: Bassett, Andrew R., et al.
Publicado: (2013)

Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice
por: Mikami, Masafumi, et al.
Publicado: (2015)

Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation
por: Fan, Di, et al.
Publicado: (2015)

CRISPR/Cas9‐mediated efficient targeted mutagenesis in grape in the first generation
por: Wang, Xianhang, et al.
Publicado: (2017)

Verification of DNA motifs in Arabidopsis using CRISPR/Cas9‐mediated mutagenesis
por: Li, Chenlong, et al.
Publicado: (2018)

Mutagenesis of seed storage protein genes in Soybean using CRISPR/Cas9
por: Li, Chenlong, et al.
Publicado: (2019)

CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry
por: Wilson, Fiona M., et al.
Publicado: (2019)

Targeted mutagenesis in ryegrass (Lolium spp.) using the CRISPR/Cas9 system
por: Zhang, Yunwei, et al.
Publicado: (2020)

CRISPR-Cas9-Based Mutagenesis of the Mucormycosis-Causing Fungus Lichtheimia corymbifera
por: Ibragimova, Sandugash, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_1hvulqe4oo8685jls1d3v5t66g