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MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1
Osteosarcoma is the most common primary bone tumour. Increasing evidence has demonstrated the pathogenic role of microRNA (miRNAs) dysregulation in tumour development. miR‐379 was previously reported to function as an oncogenic or tumour‐suppressing miRNA in a tissue‐dependent manner. However, its f...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264134/ https://www.ncbi.nlm.nih.gov/pubmed/27781416 http://dx.doi.org/10.1111/jcmm.12966 |
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author | Li, Zheng Shen, Jianxiong Chan, Matthew T. V. Wu, William Ka Kei |
author_facet | Li, Zheng Shen, Jianxiong Chan, Matthew T. V. Wu, William Ka Kei |
author_sort | Li, Zheng |
collection | PubMed |
description | Osteosarcoma is the most common primary bone tumour. Increasing evidence has demonstrated the pathogenic role of microRNA (miRNAs) dysregulation in tumour development. miR‐379 was previously reported to function as an oncogenic or tumour‐suppressing miRNA in a tissue‐dependent manner. However, its function in osteosarcoma remains unknown. In this study, we found that the expression of miR‐379 was downregulated in osteosarcoma tissues and cell lines. Further functional characterization revealed that miR‐379 suppressed osteosarcoma cell proliferation and invasion in vitro and retarded the growth of osteosarcoma xenografts in vivo. Mechanistically, PDK1 was identified as the direct target of miR‐379 in osteosarcoma, in which PDK1 expression was up‐regulated and showed inverse correlation with miR‐379. Enforced expression of PDK1 promoted osteosarcoma cell proliferation and rescued the anti‐proliferative effect of miR‐379. These data suggest that miR‐379 could function as a tumour‐suppressing miRNA via targeting PDK1 in osteosarcoma. |
format | Online Article Text |
id | pubmed-5264134 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52641342017-02-01 MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1 Li, Zheng Shen, Jianxiong Chan, Matthew T. V. Wu, William Ka Kei J Cell Mol Med Original Articles Osteosarcoma is the most common primary bone tumour. Increasing evidence has demonstrated the pathogenic role of microRNA (miRNAs) dysregulation in tumour development. miR‐379 was previously reported to function as an oncogenic or tumour‐suppressing miRNA in a tissue‐dependent manner. However, its function in osteosarcoma remains unknown. In this study, we found that the expression of miR‐379 was downregulated in osteosarcoma tissues and cell lines. Further functional characterization revealed that miR‐379 suppressed osteosarcoma cell proliferation and invasion in vitro and retarded the growth of osteosarcoma xenografts in vivo. Mechanistically, PDK1 was identified as the direct target of miR‐379 in osteosarcoma, in which PDK1 expression was up‐regulated and showed inverse correlation with miR‐379. Enforced expression of PDK1 promoted osteosarcoma cell proliferation and rescued the anti‐proliferative effect of miR‐379. These data suggest that miR‐379 could function as a tumour‐suppressing miRNA via targeting PDK1 in osteosarcoma. John Wiley and Sons Inc. 2016-10-25 2017-02 /pmc/articles/PMC5264134/ /pubmed/27781416 http://dx.doi.org/10.1111/jcmm.12966 Text en © 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Li, Zheng Shen, Jianxiong Chan, Matthew T. V. Wu, William Ka Kei MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1 |
title | MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1 |
title_full | MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1 |
title_fullStr | MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1 |
title_full_unstemmed | MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1 |
title_short | MicroRNA‐379 suppresses osteosarcoma progression by targeting PDK1 |
title_sort | microrna‐379 suppresses osteosarcoma progression by targeting pdk1 |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264134/ https://www.ncbi.nlm.nih.gov/pubmed/27781416 http://dx.doi.org/10.1111/jcmm.12966 |
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