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A quantitative approach to evaluate image quality of whole slide imaging scanners

CONTEXT: The quality of images produced by whole slide imaging (WSI) scanners has a direct influence on the readers’ performance and reliability of the clinical diagnosis. Therefore, WSI scanners should produce not only high quality but also consistent quality images. AIM: We aim to evaluate reprodu...

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Autores principales: Shrestha, Prarthana, Kneepkens, R., Vrijnsen, J., Vossen, D., Abels, E., Hulsken, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264278/
https://www.ncbi.nlm.nih.gov/pubmed/28197359
http://dx.doi.org/10.4103/2153-3539.197205
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author Shrestha, Prarthana
Kneepkens, R.
Vrijnsen, J.
Vossen, D.
Abels, E.
Hulsken, B.
author_facet Shrestha, Prarthana
Kneepkens, R.
Vrijnsen, J.
Vossen, D.
Abels, E.
Hulsken, B.
author_sort Shrestha, Prarthana
collection PubMed
description CONTEXT: The quality of images produced by whole slide imaging (WSI) scanners has a direct influence on the readers’ performance and reliability of the clinical diagnosis. Therefore, WSI scanners should produce not only high quality but also consistent quality images. AIM: We aim to evaluate reproducibility of WSI scanners based on the quality of images produced over time and among multiple scanners. The evaluation is independent of content or context of test specimen. METHODS: The ultimate judge of image quality is a pathologist, however, subjective evaluations are heavily influenced by the complexity of a case and subtle variations introduced by a scanner can be easily overlooked. Therefore, we employed a quantitative image quality assessment method based on clinically relevant parameters, such as sharpness and brightness, acquired in a survey of pathologists. The acceptable level of quality per parameter was determined in a subjective study. The evaluation of scanner reproducibility was conducted with Philips Ultra-Fast Scanners. A set of 36 HercepTest™ slides were used in three sub-studies addressing variations due to systems and time, producing 8640 test images for evaluation. RESULTS: The results showed that the majority of images in all the sub-studies are within the acceptable quality level; however, some scanners produce higher quality images more often than others. The results are independent of case types, and they match our perception of quality. CONCLUSION: The quantitative image quality assessment method was successfully applied in the HercepTest™ slides to evaluate WSI scanner reproducibility. The proposed method is generic and applicable to any other types of slide stains and scanners.
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spelling pubmed-52642782017-02-14 A quantitative approach to evaluate image quality of whole slide imaging scanners Shrestha, Prarthana Kneepkens, R. Vrijnsen, J. Vossen, D. Abels, E. Hulsken, B. J Pathol Inform Original Article CONTEXT: The quality of images produced by whole slide imaging (WSI) scanners has a direct influence on the readers’ performance and reliability of the clinical diagnosis. Therefore, WSI scanners should produce not only high quality but also consistent quality images. AIM: We aim to evaluate reproducibility of WSI scanners based on the quality of images produced over time and among multiple scanners. The evaluation is independent of content or context of test specimen. METHODS: The ultimate judge of image quality is a pathologist, however, subjective evaluations are heavily influenced by the complexity of a case and subtle variations introduced by a scanner can be easily overlooked. Therefore, we employed a quantitative image quality assessment method based on clinically relevant parameters, such as sharpness and brightness, acquired in a survey of pathologists. The acceptable level of quality per parameter was determined in a subjective study. The evaluation of scanner reproducibility was conducted with Philips Ultra-Fast Scanners. A set of 36 HercepTest™ slides were used in three sub-studies addressing variations due to systems and time, producing 8640 test images for evaluation. RESULTS: The results showed that the majority of images in all the sub-studies are within the acceptable quality level; however, some scanners produce higher quality images more often than others. The results are independent of case types, and they match our perception of quality. CONCLUSION: The quantitative image quality assessment method was successfully applied in the HercepTest™ slides to evaluate WSI scanner reproducibility. The proposed method is generic and applicable to any other types of slide stains and scanners. Medknow Publications & Media Pvt Ltd 2016-12-30 /pmc/articles/PMC5264278/ /pubmed/28197359 http://dx.doi.org/10.4103/2153-3539.197205 Text en Copyright: © 2016 Journal of Pathology Informatics http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Shrestha, Prarthana
Kneepkens, R.
Vrijnsen, J.
Vossen, D.
Abels, E.
Hulsken, B.
A quantitative approach to evaluate image quality of whole slide imaging scanners
title A quantitative approach to evaluate image quality of whole slide imaging scanners
title_full A quantitative approach to evaluate image quality of whole slide imaging scanners
title_fullStr A quantitative approach to evaluate image quality of whole slide imaging scanners
title_full_unstemmed A quantitative approach to evaluate image quality of whole slide imaging scanners
title_short A quantitative approach to evaluate image quality of whole slide imaging scanners
title_sort quantitative approach to evaluate image quality of whole slide imaging scanners
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264278/
https://www.ncbi.nlm.nih.gov/pubmed/28197359
http://dx.doi.org/10.4103/2153-3539.197205
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