A new micro swim-up procedure for sperm preparation in ICSI treatments: preliminary microbiological testing

OBJECTIVE: This study aimed to assess the levels of microbial contamination in semen samples before and after the micro swim-up (MSU) procedure in intra-cytoplasmic sperm injection (ICSI). The new method is an upgrade to the classic wash swim-up procedure. METHODS: Semen analysis and microbiological...

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Detalles Bibliográficos
Autores principales: Palini, Simone, Primiterra, Mariangela, De Stefani, Silvia, Pedna, Maria Federica, Sparacino, Monica, Farabegoli, Patrizia, Benedetti, Serena, Bulletti, Carlo, Sambri, Vittorio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Assisted Reproduction 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264371/
https://www.ncbi.nlm.nih.gov/pubmed/27584599
http://dx.doi.org/10.5935/1518-0557.20160023
Descripción
Sumario:OBJECTIVE: This study aimed to assess the levels of microbial contamination in semen samples before and after the micro swim-up (MSU) procedure in intra-cytoplasmic sperm injection (ICSI). The new method is an upgrade to the classic wash swim-up procedure. METHODS: Semen analysis and microbiological tests were carried out before and after the MSU procedure. A total of twenty semen samples were analyzed. RESULTS: Pathogens were observed in semen samples only before MSU and never after ICSI. Microbiological tests revealed a large prevalence of gram-positive cocci [Staphylococcus spp. (n=16, 80%) and viridans streptococci (n=10, 50%)]. The results of this study indicate that direct MSU in ICSI improved the ICSI workflow. CONCLUSION: The new workflow is faster and more affordable, and is likely to prevent infection problems that could arise from the normal microbial flora of the semen.

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