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Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn(2+) Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots
Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn(2+)) in treated roots of A...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264391/ https://www.ncbi.nlm.nih.gov/pubmed/28120857 http://dx.doi.org/10.1038/srep40685 |
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author | Ahmed, Bilal Dwivedi, Sourabh Abdin, Malik Zainul Azam, Ameer Al-Shaeri, Majed Khan, Mohammad Saghir Saquib, Quaiser Al-Khedhairy, Abdulaziz A. Musarrat, Javed |
author_facet | Ahmed, Bilal Dwivedi, Sourabh Abdin, Malik Zainul Azam, Ameer Al-Shaeri, Majed Khan, Mohammad Saghir Saquib, Quaiser Al-Khedhairy, Abdulaziz A. Musarrat, Javed |
author_sort | Ahmed, Bilal |
collection | PubMed |
description | Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn(2+)) in treated roots of Allium cepa, under hydroponic conditions. ZnO-NPs were characterized by UV-visible, XRD, FT-IR spectroscopy and TEM analyses. Bulbs of A. cepa exposed to ZnO-NPs (25.5 nm) for 12 h exhibited significant decrease (23 ± 8.7%) in % mitotic index and increase in chromosomal aberrations (18 ± 7.6%), in a dose-dependent manner. Transmission electron microcopy and FT-IR data suggested surface attachment, internalization and biomolecular intervention of ZnO-NPs in root cells, respectively. The levels of TBARS and antioxidant enzymes were found to be significantly greater in treated root cells vis-à-vis untreated control. Furthermore, dose-dependent increase in ROS production and alterations in ΔΨm were observed in treated roots. FT-IR analysis of root tissues demonstrated symmetric and asymmetric P=O stretching of >PO(2)(−) at 1240 cm(−1) and stretching of C-O ribose at 1060 cm(−1), suggestive of nuclear damage. Overall, the results elucidated A. cepa, as a good model for assessment of cytotoxicity and oxidative DNA damage with ZnO-NPs and Zn(2+) in plants. |
format | Online Article Text |
id | pubmed-5264391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52643912017-01-30 Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn(2+) Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots Ahmed, Bilal Dwivedi, Sourabh Abdin, Malik Zainul Azam, Ameer Al-Shaeri, Majed Khan, Mohammad Saghir Saquib, Quaiser Al-Khedhairy, Abdulaziz A. Musarrat, Javed Sci Rep Article Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn(2+)) in treated roots of Allium cepa, under hydroponic conditions. ZnO-NPs were characterized by UV-visible, XRD, FT-IR spectroscopy and TEM analyses. Bulbs of A. cepa exposed to ZnO-NPs (25.5 nm) for 12 h exhibited significant decrease (23 ± 8.7%) in % mitotic index and increase in chromosomal aberrations (18 ± 7.6%), in a dose-dependent manner. Transmission electron microcopy and FT-IR data suggested surface attachment, internalization and biomolecular intervention of ZnO-NPs in root cells, respectively. The levels of TBARS and antioxidant enzymes were found to be significantly greater in treated root cells vis-à-vis untreated control. Furthermore, dose-dependent increase in ROS production and alterations in ΔΨm were observed in treated roots. FT-IR analysis of root tissues demonstrated symmetric and asymmetric P=O stretching of >PO(2)(−) at 1240 cm(−1) and stretching of C-O ribose at 1060 cm(−1), suggestive of nuclear damage. Overall, the results elucidated A. cepa, as a good model for assessment of cytotoxicity and oxidative DNA damage with ZnO-NPs and Zn(2+) in plants. Nature Publishing Group 2017-01-25 /pmc/articles/PMC5264391/ /pubmed/28120857 http://dx.doi.org/10.1038/srep40685 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ahmed, Bilal Dwivedi, Sourabh Abdin, Malik Zainul Azam, Ameer Al-Shaeri, Majed Khan, Mohammad Saghir Saquib, Quaiser Al-Khedhairy, Abdulaziz A. Musarrat, Javed Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn(2+) Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots |
title | Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn(2+) Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots |
title_full | Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn(2+) Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots |
title_fullStr | Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn(2+) Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots |
title_full_unstemmed | Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn(2+) Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots |
title_short | Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn(2+) Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots |
title_sort | mitochondrial and chromosomal damage induced by oxidative stress in zn(2+) ions, zno-bulk and zno-nps treated allium cepa roots |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264391/ https://www.ncbi.nlm.nih.gov/pubmed/28120857 http://dx.doi.org/10.1038/srep40685 |
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