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Selection of chemically defined media for CHO cell fed-batch culture processes

Two CHO cell clones derived from the same parental CHO(BC®) cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher am...

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Autores principales: Pan, Xiao, Streefland, Mathieu, Dalm, Ciska, Wijffels, René H., Martens, Dirk E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264622/
https://www.ncbi.nlm.nih.gov/pubmed/27900626
http://dx.doi.org/10.1007/s10616-016-0036-5
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author Pan, Xiao
Streefland, Mathieu
Dalm, Ciska
Wijffels, René H.
Martens, Dirk E.
author_facet Pan, Xiao
Streefland, Mathieu
Dalm, Ciska
Wijffels, René H.
Martens, Dirk E.
author_sort Pan, Xiao
collection PubMed
description Two CHO cell clones derived from the same parental CHO(BC®) cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher amino acid feeding did not always lead to higher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and production rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B. The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary phase. Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B). The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10616-016-0036-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-52646222017-02-07 Selection of chemically defined media for CHO cell fed-batch culture processes Pan, Xiao Streefland, Mathieu Dalm, Ciska Wijffels, René H. Martens, Dirk E. Cytotechnology Original Article Two CHO cell clones derived from the same parental CHO(BC®) cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher amino acid feeding did not always lead to higher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and production rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B. The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary phase. Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B). The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10616-016-0036-5) contains supplementary material, which is available to authorized users. Springer Netherlands 2016-11-29 2017-02 /pmc/articles/PMC5264622/ /pubmed/27900626 http://dx.doi.org/10.1007/s10616-016-0036-5 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Pan, Xiao
Streefland, Mathieu
Dalm, Ciska
Wijffels, René H.
Martens, Dirk E.
Selection of chemically defined media for CHO cell fed-batch culture processes
title Selection of chemically defined media for CHO cell fed-batch culture processes
title_full Selection of chemically defined media for CHO cell fed-batch culture processes
title_fullStr Selection of chemically defined media for CHO cell fed-batch culture processes
title_full_unstemmed Selection of chemically defined media for CHO cell fed-batch culture processes
title_short Selection of chemically defined media for CHO cell fed-batch culture processes
title_sort selection of chemically defined media for cho cell fed-batch culture processes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264622/
https://www.ncbi.nlm.nih.gov/pubmed/27900626
http://dx.doi.org/10.1007/s10616-016-0036-5
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