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Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report

Pre-implantation genetic diagnosis (PGD) or screening (PGS) technology, has emerged and developed in the past few years, benefiting couples as it allows the selection and transfer of healthy embryos during IVF treatments. These techniques can be performed in oocytes (polar-body biopsy) or embryos (b...

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Autores principales: Guimarães, Fernando, Roque, Matheus, Valle, Marcello, Kostolias, Alessandra, de Azevedo, Rodrigo A, Martinhago, Ciro D, Sampaio, Marcos, Geber, Selmo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Assisted Reproduction 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5265627/
https://www.ncbi.nlm.nih.gov/pubmed/28050963
http://dx.doi.org/10.5935/1518-0557.20160049
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author Guimarães, Fernando
Roque, Matheus
Valle, Marcello
Kostolias, Alessandra
de Azevedo, Rodrigo A
Martinhago, Ciro D
Sampaio, Marcos
Geber, Selmo
author_facet Guimarães, Fernando
Roque, Matheus
Valle, Marcello
Kostolias, Alessandra
de Azevedo, Rodrigo A
Martinhago, Ciro D
Sampaio, Marcos
Geber, Selmo
author_sort Guimarães, Fernando
collection PubMed
description Pre-implantation genetic diagnosis (PGD) or screening (PGS) technology, has emerged and developed in the past few years, benefiting couples as it allows the selection and transfer of healthy embryos during IVF treatments. These techniques can be performed in oocytes (polar-body biopsy) or embryos (blastomere or trophectoderm biopsy). In this case report, we describe the first two live births to be published in Brazil after a polar-body (PB) biopsy. In case 1, a 42-year-old was submitted to PB biopsy with PGS due to advanced maternal age and poor ovarian reserve. Five MII oocytes underwent first and second polar body biopsy and four cleavage embryos were cryopreserved. The PGS analysis resulted in two euploid embryos (next generation sequence). A frozen-thawed embryo transfer (FET) was performed after endometrial priming and a healthy baby was delivered after a cesarean section (37 weeks, female, 3390g, 47.5 cm). In case 2, a 40-year old patient with balanced translocation and poor ovarian response was submitted to PB biopsy. Two MII oocytes underwent first and second polar body biopsy and two embryos were cryopreserved in cleavage stage. The analysis resulted in one euploid embryo that was transferred after endometrial priming. A preterm healthy baby (34 weeks, female, 2100g, 40 cm) was delivered via cesarean section. In conclusion, although the blastocyst biopsy is the norm when performing PGS/PGD during IVF treatments, other alternatives (as PB biopsy) should be considered in some specific situations.
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spelling pubmed-52656272017-03-23 Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report Guimarães, Fernando Roque, Matheus Valle, Marcello Kostolias, Alessandra de Azevedo, Rodrigo A Martinhago, Ciro D Sampaio, Marcos Geber, Selmo JBRA Assist Reprod Case Report Pre-implantation genetic diagnosis (PGD) or screening (PGS) technology, has emerged and developed in the past few years, benefiting couples as it allows the selection and transfer of healthy embryos during IVF treatments. These techniques can be performed in oocytes (polar-body biopsy) or embryos (blastomere or trophectoderm biopsy). In this case report, we describe the first two live births to be published in Brazil after a polar-body (PB) biopsy. In case 1, a 42-year-old was submitted to PB biopsy with PGS due to advanced maternal age and poor ovarian reserve. Five MII oocytes underwent first and second polar body biopsy and four cleavage embryos were cryopreserved. The PGS analysis resulted in two euploid embryos (next generation sequence). A frozen-thawed embryo transfer (FET) was performed after endometrial priming and a healthy baby was delivered after a cesarean section (37 weeks, female, 3390g, 47.5 cm). In case 2, a 40-year old patient with balanced translocation and poor ovarian response was submitted to PB biopsy. Two MII oocytes underwent first and second polar body biopsy and two embryos were cryopreserved in cleavage stage. The analysis resulted in one euploid embryo that was transferred after endometrial priming. A preterm healthy baby (34 weeks, female, 2100g, 40 cm) was delivered via cesarean section. In conclusion, although the blastocyst biopsy is the norm when performing PGS/PGD during IVF treatments, other alternatives (as PB biopsy) should be considered in some specific situations. Brazilian Society of Assisted Reproduction 2016 /pmc/articles/PMC5265627/ /pubmed/28050963 http://dx.doi.org/10.5935/1518-0557.20160049 Text en http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Case Report
Guimarães, Fernando
Roque, Matheus
Valle, Marcello
Kostolias, Alessandra
de Azevedo, Rodrigo A
Martinhago, Ciro D
Sampaio, Marcos
Geber, Selmo
Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report
title Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report
title_full Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report
title_fullStr Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report
title_full_unstemmed Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report
title_short Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report
title_sort live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5265627/
https://www.ncbi.nlm.nih.gov/pubmed/28050963
http://dx.doi.org/10.5935/1518-0557.20160049
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