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Characterization of Caveola-Vesicle Complexes (CVCs) Protein, PHIST/CVC-81(95) in Plasmodium vivax
Plasmodium vivax produces numerous caveola-vesicle complex (CVC) structures beneath the membrane of infected erythrocytes. Recently, a member helical interspersed subtelomeric (PHIST) superfamily protein, PcyPHIST/CVC-81(95), was identified as CVCs-associated protein in Plasmodium cynomolgi and esse...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Parasitology and Tropical Medicine
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5266361/ https://www.ncbi.nlm.nih.gov/pubmed/28095657 http://dx.doi.org/10.3347/kjp.2016.54.6.725 |
Sumario: | Plasmodium vivax produces numerous caveola-vesicle complex (CVC) structures beneath the membrane of infected erythrocytes. Recently, a member helical interspersed subtelomeric (PHIST) superfamily protein, PcyPHIST/CVC-81(95), was identified as CVCs-associated protein in Plasmodium cynomolgi and essential for survival of this parasite. Very little information has been documented to date about PHIST/CVC-81(95) protein in P. vivax. In this study, the recombinant PvPHIST/CVC-81(95) N and C termini were expressed, and immunoreactivity was assessed using confirmed vivax malaria patients sera by protein microarray. The subcellular localization of PvPHIST/CVC-81(95) N and C termini in blood stage parasites was also determined. The antigenicity of recombinant PvPHIST/CVC-81(95) N and C terminal proteins were analyzed by using serum samples from the Republic of Korea. The results showed that immunoreactivities to these proteins had 61% and 43% sensitivity and 96.9% and 93.8% specificity, respectively. The N terminal of PvPHIST/CVC-81(95) which contains transmembrane domain and export motif (PEXEL; RxLxE/Q/D) produced CVCs location throughout the erythrocytic-stage parasites. However, no fluorescence was detected with antibodies against C terminal fragment of PvPHIST/CVC-81(95). These results suggest that the PvPHIST/CVC-81(95) is localized on the CVCs and may be immunogenic in natural infection of P. vivax. |
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