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Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata
Lemnaceae (commonly called duckweed) is an aquatic plant ideal for quantitative analysis in plant sciences. Several species of this family represent the smallest and fastest growing flowering plants. Different ecotypes of the same species vary in their biochemical and physiological properties. Thus,...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5266846/ https://www.ncbi.nlm.nih.gov/pubmed/28168191 http://dx.doi.org/10.1155/2017/5196763 |
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author | Feng, Bo Fang, Yang Xu, Zhibin Xiang, Chao Zhou, Chunhong Jiang, Fei Wang, Tao Zhao, Hai |
author_facet | Feng, Bo Fang, Yang Xu, Zhibin Xiang, Chao Zhou, Chunhong Jiang, Fei Wang, Tao Zhao, Hai |
author_sort | Feng, Bo |
collection | PubMed |
description | Lemnaceae (commonly called duckweed) is an aquatic plant ideal for quantitative analysis in plant sciences. Several species of this family represent the smallest and fastest growing flowering plants. Different ecotypes of the same species vary in their biochemical and physiological properties. Thus, selecting of desirable ecotypes of a species is very important. Here, we developed a simple and rapid molecular identification system for Spirodela polyrhiza and Landoltia punctata based on the sequence polymorphism. First, several pairs of primers were designed and three markers were selected as good for identification. After PCR amplification, DNA fragments (the combination of three PCR products) in different duckweeds were detected using capillary electrophoresis. The high-resolution capillary electrophoresis displayed high identity to the sequencing results. The combination of the PCR products containing several DNA fragments highly improved the identification frequency. These results indicate that this method is not only good for interspecies identification but also ideal for intraspecies distinguishing. Meanwhile, 11 haplotypes were found in both the S. polyrhiza and L. punctata ecotypes. The results suggest that this marker system is useful for large-scale identification of duckweed and for the screening of desirable ecotypes to improve the diverse usage in duckweed utilization. |
format | Online Article Text |
id | pubmed-5266846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52668462017-02-06 Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata Feng, Bo Fang, Yang Xu, Zhibin Xiang, Chao Zhou, Chunhong Jiang, Fei Wang, Tao Zhao, Hai Int J Genomics Research Article Lemnaceae (commonly called duckweed) is an aquatic plant ideal for quantitative analysis in plant sciences. Several species of this family represent the smallest and fastest growing flowering plants. Different ecotypes of the same species vary in their biochemical and physiological properties. Thus, selecting of desirable ecotypes of a species is very important. Here, we developed a simple and rapid molecular identification system for Spirodela polyrhiza and Landoltia punctata based on the sequence polymorphism. First, several pairs of primers were designed and three markers were selected as good for identification. After PCR amplification, DNA fragments (the combination of three PCR products) in different duckweeds were detected using capillary electrophoresis. The high-resolution capillary electrophoresis displayed high identity to the sequencing results. The combination of the PCR products containing several DNA fragments highly improved the identification frequency. These results indicate that this method is not only good for interspecies identification but also ideal for intraspecies distinguishing. Meanwhile, 11 haplotypes were found in both the S. polyrhiza and L. punctata ecotypes. The results suggest that this marker system is useful for large-scale identification of duckweed and for the screening of desirable ecotypes to improve the diverse usage in duckweed utilization. Hindawi Publishing Corporation 2017 2017-01-12 /pmc/articles/PMC5266846/ /pubmed/28168191 http://dx.doi.org/10.1155/2017/5196763 Text en Copyright © 2017 Bo Feng et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Feng, Bo Fang, Yang Xu, Zhibin Xiang, Chao Zhou, Chunhong Jiang, Fei Wang, Tao Zhao, Hai Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
title | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
title_full | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
title_fullStr | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
title_full_unstemmed | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
title_short | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
title_sort | development of a new marker system for identification of spirodela polyrhiza and landoltia punctata |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5266846/ https://www.ncbi.nlm.nih.gov/pubmed/28168191 http://dx.doi.org/10.1155/2017/5196763 |
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