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Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection

A simple and rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method has been developed and validated for simultaneous quantification of five antiemetic agents in infusion samples: dexamethasone, ondansetron, granisetron, tropisetron, and azasetron. The chromatograph...

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Autores principales: Chen, Fu-chao, Wang, Lin-hai, Guo, Jun, Shi, Xiao-ya, Fang, Bao-xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5266853/
https://www.ncbi.nlm.nih.gov/pubmed/28168082
http://dx.doi.org/10.1155/2017/6749087
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author Chen, Fu-chao
Wang, Lin-hai
Guo, Jun
Shi, Xiao-ya
Fang, Bao-xia
author_facet Chen, Fu-chao
Wang, Lin-hai
Guo, Jun
Shi, Xiao-ya
Fang, Bao-xia
author_sort Chen, Fu-chao
collection PubMed
description A simple and rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method has been developed and validated for simultaneous quantification of five antiemetic agents in infusion samples: dexamethasone, ondansetron, granisetron, tropisetron, and azasetron. The chromatographic separation was achieved on a Phenomenex C(18) column (4.6 mm × 150 mm, 5 μm) using acetonitrile-50 mM KH(2)PO(4) buffer-triethylamine (25 : 74 : 1; v/v; pH 4.0). Flow rate was 1.0 mL/min with a column temperature of 30°C. Validation of the method was made in terms of specificity, linearity, accuracy, and intra- and interday precision, as well as quantification and detection limits. The developed method can be used in the laboratory to routinely quantify dexamethasone, ondansetron, granisetron, tropisetron, and azasetron simultaneously and to evaluate the physicochemical stability of referred drugs in mixtures for endovenous use.
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spelling pubmed-52668532017-02-06 Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection Chen, Fu-chao Wang, Lin-hai Guo, Jun Shi, Xiao-ya Fang, Bao-xia J Anal Methods Chem Research Article A simple and rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method has been developed and validated for simultaneous quantification of five antiemetic agents in infusion samples: dexamethasone, ondansetron, granisetron, tropisetron, and azasetron. The chromatographic separation was achieved on a Phenomenex C(18) column (4.6 mm × 150 mm, 5 μm) using acetonitrile-50 mM KH(2)PO(4) buffer-triethylamine (25 : 74 : 1; v/v; pH 4.0). Flow rate was 1.0 mL/min with a column temperature of 30°C. Validation of the method was made in terms of specificity, linearity, accuracy, and intra- and interday precision, as well as quantification and detection limits. The developed method can be used in the laboratory to routinely quantify dexamethasone, ondansetron, granisetron, tropisetron, and azasetron simultaneously and to evaluate the physicochemical stability of referred drugs in mixtures for endovenous use. Hindawi Publishing Corporation 2017 2017-01-11 /pmc/articles/PMC5266853/ /pubmed/28168082 http://dx.doi.org/10.1155/2017/6749087 Text en Copyright © 2017 Fu-chao Chen et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Fu-chao
Wang, Lin-hai
Guo, Jun
Shi, Xiao-ya
Fang, Bao-xia
Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection
title Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection
title_full Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection
title_fullStr Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection
title_full_unstemmed Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection
title_short Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection
title_sort simultaneous determination of dexamethasone, ondansetron, granisetron, tropisetron, and azasetron in infusion samples by hplc with dad detection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5266853/
https://www.ncbi.nlm.nih.gov/pubmed/28168082
http://dx.doi.org/10.1155/2017/6749087
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