Cargando…
Antioxidants of Phyllanthus emblica L. Bark Extract Provide Hepatoprotection against Ethanol-Induced Hepatic Damage: A Comparison with Silymarin
Phyllanthus emblica L. (amla) has been used in Ayurveda as a potent rasayan for treatment of hepatic disorders. Most of the pharmacological studies, however, are largely focused on PE fruit, while the rest of the parts of PE, particularly, bark, remain underinvestigated. Therefore, we aimed to inves...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267079/ https://www.ncbi.nlm.nih.gov/pubmed/28168009 http://dx.doi.org/10.1155/2017/3876040 |
_version_ | 1782500577002913792 |
---|---|
author | Chaphalkar, Renuka Apte, Kishori G. Talekar, Yogesh Ojha, Shreesh Kumar Nandave, Mukesh |
author_facet | Chaphalkar, Renuka Apte, Kishori G. Talekar, Yogesh Ojha, Shreesh Kumar Nandave, Mukesh |
author_sort | Chaphalkar, Renuka |
collection | PubMed |
description | Phyllanthus emblica L. (amla) has been used in Ayurveda as a potent rasayan for treatment of hepatic disorders. Most of the pharmacological studies, however, are largely focused on PE fruit, while the rest of the parts of PE, particularly, bark, remain underinvestigated. Therefore, we aimed to investigate the protective effect of the hydroalcoholic extract of Phyllanthus emblica bark (PEE) in ethanol-induced hepatotoxicity model in rats. Total phenolic, flavonoid, and tannin content and in vitro antioxidant activities were determined by using H(2)O(2) scavenging and ABTS decolorization assays. Our results showed that PEE was rich in total phenols (99.523 ± 1.91 mg GAE/g), total flavonoids (389.33 ± 1.25 mg quercetin hydrate/g), and total tannins (310 ± 0.21 mg catechin/g), which clearly support its strong antioxidant potential. HPTLC-based quantitative analysis revealed the presence of the potent antioxidants gallic acid (25.05 mg/g) and ellagic acid (13.31 mg/g). Moreover, one-month PEE treatment (500 and 1000 mg/kg, p.o.) followed by 30-day 70% ethanol (10 mL/kg) administration showed hepatoprotection as evidenced by significant restoration of ALT (p < 0.01), AST (p < 0.001), ALP (p < 0.05), and TP (p < 0.001) and further confirmed by liver histopathology. PEE-mediated hepatoprotection could be due to its free radical scavenging and antioxidant activity that may be ascribed to its antioxidant components, namely, ellagic acid and gallic acid. Thus, the results of the present study support the therapeutic claims made in Ayurveda about Phyllanthus emblica. |
format | Online Article Text |
id | pubmed-5267079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52670792017-02-06 Antioxidants of Phyllanthus emblica L. Bark Extract Provide Hepatoprotection against Ethanol-Induced Hepatic Damage: A Comparison with Silymarin Chaphalkar, Renuka Apte, Kishori G. Talekar, Yogesh Ojha, Shreesh Kumar Nandave, Mukesh Oxid Med Cell Longev Research Article Phyllanthus emblica L. (amla) has been used in Ayurveda as a potent rasayan for treatment of hepatic disorders. Most of the pharmacological studies, however, are largely focused on PE fruit, while the rest of the parts of PE, particularly, bark, remain underinvestigated. Therefore, we aimed to investigate the protective effect of the hydroalcoholic extract of Phyllanthus emblica bark (PEE) in ethanol-induced hepatotoxicity model in rats. Total phenolic, flavonoid, and tannin content and in vitro antioxidant activities were determined by using H(2)O(2) scavenging and ABTS decolorization assays. Our results showed that PEE was rich in total phenols (99.523 ± 1.91 mg GAE/g), total flavonoids (389.33 ± 1.25 mg quercetin hydrate/g), and total tannins (310 ± 0.21 mg catechin/g), which clearly support its strong antioxidant potential. HPTLC-based quantitative analysis revealed the presence of the potent antioxidants gallic acid (25.05 mg/g) and ellagic acid (13.31 mg/g). Moreover, one-month PEE treatment (500 and 1000 mg/kg, p.o.) followed by 30-day 70% ethanol (10 mL/kg) administration showed hepatoprotection as evidenced by significant restoration of ALT (p < 0.01), AST (p < 0.001), ALP (p < 0.05), and TP (p < 0.001) and further confirmed by liver histopathology. PEE-mediated hepatoprotection could be due to its free radical scavenging and antioxidant activity that may be ascribed to its antioxidant components, namely, ellagic acid and gallic acid. Thus, the results of the present study support the therapeutic claims made in Ayurveda about Phyllanthus emblica. Hindawi Publishing Corporation 2017 2017-01-12 /pmc/articles/PMC5267079/ /pubmed/28168009 http://dx.doi.org/10.1155/2017/3876040 Text en Copyright © 2017 Renuka Chaphalkar et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chaphalkar, Renuka Apte, Kishori G. Talekar, Yogesh Ojha, Shreesh Kumar Nandave, Mukesh Antioxidants of Phyllanthus emblica L. Bark Extract Provide Hepatoprotection against Ethanol-Induced Hepatic Damage: A Comparison with Silymarin |
title | Antioxidants of Phyllanthus emblica L. Bark Extract Provide Hepatoprotection against Ethanol-Induced Hepatic Damage: A Comparison with Silymarin |
title_full | Antioxidants of Phyllanthus emblica L. Bark Extract Provide Hepatoprotection against Ethanol-Induced Hepatic Damage: A Comparison with Silymarin |
title_fullStr | Antioxidants of Phyllanthus emblica L. Bark Extract Provide Hepatoprotection against Ethanol-Induced Hepatic Damage: A Comparison with Silymarin |
title_full_unstemmed | Antioxidants of Phyllanthus emblica L. Bark Extract Provide Hepatoprotection against Ethanol-Induced Hepatic Damage: A Comparison with Silymarin |
title_short | Antioxidants of Phyllanthus emblica L. Bark Extract Provide Hepatoprotection against Ethanol-Induced Hepatic Damage: A Comparison with Silymarin |
title_sort | antioxidants of phyllanthus emblica l. bark extract provide hepatoprotection against ethanol-induced hepatic damage: a comparison with silymarin |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267079/ https://www.ncbi.nlm.nih.gov/pubmed/28168009 http://dx.doi.org/10.1155/2017/3876040 |
work_keys_str_mv | AT chaphalkarrenuka antioxidantsofphyllanthusemblicalbarkextractprovidehepatoprotectionagainstethanolinducedhepaticdamageacomparisonwithsilymarin AT aptekishorig antioxidantsofphyllanthusemblicalbarkextractprovidehepatoprotectionagainstethanolinducedhepaticdamageacomparisonwithsilymarin AT talekaryogesh antioxidantsofphyllanthusemblicalbarkextractprovidehepatoprotectionagainstethanolinducedhepaticdamageacomparisonwithsilymarin AT ojhashreeshkumar antioxidantsofphyllanthusemblicalbarkextractprovidehepatoprotectionagainstethanolinducedhepaticdamageacomparisonwithsilymarin AT nandavemukesh antioxidantsofphyllanthusemblicalbarkextractprovidehepatoprotectionagainstethanolinducedhepaticdamageacomparisonwithsilymarin |