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Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams

Tissue samples from renal cell carcinoma patients were analyzed by electrospray droplet ion beam-induced secondary ion mass spectrometry (EDI/SIMS). Positively- and negatively-charged secondary ions were measured for the cancerous and noncancerous regions of the tissue samples. Although specific can...

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Autores principales: Ninomiya, Satoshi, Yoshimura, Kentaro, Chen, Lee Chuin, Takeda, Sen, Hiraoka, Kenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Mass Spectrometry Society of Japan 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267555/
https://www.ncbi.nlm.nih.gov/pubmed/28149705
http://dx.doi.org/10.5702/massspectrometry.A0053
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author Ninomiya, Satoshi
Yoshimura, Kentaro
Chen, Lee Chuin
Takeda, Sen
Hiraoka, Kenzo
author_facet Ninomiya, Satoshi
Yoshimura, Kentaro
Chen, Lee Chuin
Takeda, Sen
Hiraoka, Kenzo
author_sort Ninomiya, Satoshi
collection PubMed
description Tissue samples from renal cell carcinoma patients were analyzed by electrospray droplet ion beam-induced secondary ion mass spectrometry (EDI/SIMS). Positively- and negatively-charged secondary ions were measured for the cancerous and noncancerous regions of the tissue samples. Although specific cancerous species could not be found in both the positive and negative secondary ion spectra, the spectra of the cancerous and noncancerous tissues presented different trends. For instance, in the m/z range of 500–800 of the positive secondary ion spectra for the cancerous tissues, the intensities for several m/z values were lower than those of the m/z+2 peaks (indicating one double bond loss for the species), whereas, for the noncancerous tissues, the inverse trend was obtained. The tandem mass spectrometry (MS/MS) was also performed on the tissue samples using probe electrospray ionization (PESI), and some molecular ions produced by PESI were found to be fragmented into the ions observed in EDI/SIMS analysis. When the positive secondary ion spectra produced by EDI/SIMS were analyzed by principal component analysis, the results for cancerous and noncancerous tissues were separated. The EDI/SIMS method can be applied to distinguish between a cancerous and a noncancerous area with high probability.
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spelling pubmed-52675552017-02-02 Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams Ninomiya, Satoshi Yoshimura, Kentaro Chen, Lee Chuin Takeda, Sen Hiraoka, Kenzo Mass Spectrom (Tokyo) Original Article Tissue samples from renal cell carcinoma patients were analyzed by electrospray droplet ion beam-induced secondary ion mass spectrometry (EDI/SIMS). Positively- and negatively-charged secondary ions were measured for the cancerous and noncancerous regions of the tissue samples. Although specific cancerous species could not be found in both the positive and negative secondary ion spectra, the spectra of the cancerous and noncancerous tissues presented different trends. For instance, in the m/z range of 500–800 of the positive secondary ion spectra for the cancerous tissues, the intensities for several m/z values were lower than those of the m/z+2 peaks (indicating one double bond loss for the species), whereas, for the noncancerous tissues, the inverse trend was obtained. The tandem mass spectrometry (MS/MS) was also performed on the tissue samples using probe electrospray ionization (PESI), and some molecular ions produced by PESI were found to be fragmented into the ions observed in EDI/SIMS analysis. When the positive secondary ion spectra produced by EDI/SIMS were analyzed by principal component analysis, the results for cancerous and noncancerous tissues were separated. The EDI/SIMS method can be applied to distinguish between a cancerous and a noncancerous area with high probability. The Mass Spectrometry Society of Japan 2017 2017-01-27 /pmc/articles/PMC5267555/ /pubmed/28149705 http://dx.doi.org/10.5702/massspectrometry.A0053 Text en Copyright © 2017 Satoshi Ninomiya, Kentaro Yoshimura, Lee Chuin Chen, Sen Takeda, and Kenzo Hiraoka. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article distributed under the terms of Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. For permission to reuse copyrighted content from this publication, please go to www.copyright.com (http://www.copyright.com) , or contact Copyright Clearance Center, 222 Rosewood Drive, Danvers, MA 01923
spellingShingle Original Article
Ninomiya, Satoshi
Yoshimura, Kentaro
Chen, Lee Chuin
Takeda, Sen
Hiraoka, Kenzo
Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams
title Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams
title_full Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams
title_fullStr Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams
title_full_unstemmed Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams
title_short Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams
title_sort secondary ion mass spectrometry analysis of renal cell carcinoma with electrospray droplet ion beams
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267555/
https://www.ncbi.nlm.nih.gov/pubmed/28149705
http://dx.doi.org/10.5702/massspectrometry.A0053
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