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Function annotation of peptides generated from the non-coding regions of D. melanogaster genome

De novo emergence of genes is the most fundamental form of genetic diversity that is attracting the attention of the scientific community. Identification of short open reading frames (sORFs) from the non-coding regions of different genomes has been leading this thought recently. The coding potential...

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Autores principales: Deepthi, Varughese, V. I. Nair, Vineetha, Thomas, Vipin, Raj, Navya, P. Ramakrishnan, Shidhi, Khan, Juveria, Kaushik, Monika, K. Dhar, Pawan, S. Nair, Achuthsankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267965/
https://www.ncbi.nlm.nih.gov/pubmed/28149056
http://dx.doi.org/10.6026/97320630012202
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author Deepthi, Varughese
V. I. Nair, Vineetha
Thomas, Vipin
Raj, Navya
P. Ramakrishnan, Shidhi
Khan, Juveria
Kaushik, Monika
K. Dhar, Pawan
S. Nair, Achuthsankar
author_facet Deepthi, Varughese
V. I. Nair, Vineetha
Thomas, Vipin
Raj, Navya
P. Ramakrishnan, Shidhi
Khan, Juveria
Kaushik, Monika
K. Dhar, Pawan
S. Nair, Achuthsankar
author_sort Deepthi, Varughese
collection PubMed
description De novo emergence of genes is the most fundamental form of genetic diversity that is attracting the attention of the scientific community. Identification of short open reading frames (sORFs) from the non-coding regions of different genomes has been leading this thought recently. The coding potential of these newly identified sORFs have been investigated through experimental and computational approaches in recent studies. In the present work we have tried to make peptides from intergenic sequences of D. melanogaster genome leading to therapeutic applications. Towards this goal of making novel peptides from non-coding genome, we have found strong computational evidence of 145 peptides with conformational stability from the intergenic sequences of D. melanogaster. The structure of these completely unique peptides was predicted using ab initio method. The function annotation of these peptides was carried out using this structural information. The newly generated proteins were categorised as DNA/Protein/ion binding proteins, electron transporters and a very few as enzymes too. Experimental studies can certainly provide validations to these preliminary findings. This work provides further evidence of untapped potential of non-coding genome.
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spelling pubmed-52679652017-02-01 Function annotation of peptides generated from the non-coding regions of D. melanogaster genome Deepthi, Varughese V. I. Nair, Vineetha Thomas, Vipin Raj, Navya P. Ramakrishnan, Shidhi Khan, Juveria Kaushik, Monika K. Dhar, Pawan S. Nair, Achuthsankar Bioinformation Hypothesis De novo emergence of genes is the most fundamental form of genetic diversity that is attracting the attention of the scientific community. Identification of short open reading frames (sORFs) from the non-coding regions of different genomes has been leading this thought recently. The coding potential of these newly identified sORFs have been investigated through experimental and computational approaches in recent studies. In the present work we have tried to make peptides from intergenic sequences of D. melanogaster genome leading to therapeutic applications. Towards this goal of making novel peptides from non-coding genome, we have found strong computational evidence of 145 peptides with conformational stability from the intergenic sequences of D. melanogaster. The structure of these completely unique peptides was predicted using ab initio method. The function annotation of these peptides was carried out using this structural information. The newly generated proteins were categorised as DNA/Protein/ion binding proteins, electron transporters and a very few as enzymes too. Experimental studies can certainly provide validations to these preliminary findings. This work provides further evidence of untapped potential of non-coding genome. Biomedical Informatics 2016-06-15 /pmc/articles/PMC5267965/ /pubmed/28149056 http://dx.doi.org/10.6026/97320630012202 Text en © 2016 Biomedical Informatics This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
spellingShingle Hypothesis
Deepthi, Varughese
V. I. Nair, Vineetha
Thomas, Vipin
Raj, Navya
P. Ramakrishnan, Shidhi
Khan, Juveria
Kaushik, Monika
K. Dhar, Pawan
S. Nair, Achuthsankar
Function annotation of peptides generated from the non-coding regions of D. melanogaster genome
title Function annotation of peptides generated from the non-coding regions of D. melanogaster genome
title_full Function annotation of peptides generated from the non-coding regions of D. melanogaster genome
title_fullStr Function annotation of peptides generated from the non-coding regions of D. melanogaster genome
title_full_unstemmed Function annotation of peptides generated from the non-coding regions of D. melanogaster genome
title_short Function annotation of peptides generated from the non-coding regions of D. melanogaster genome
title_sort function annotation of peptides generated from the non-coding regions of d. melanogaster genome
topic Hypothesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267965/
https://www.ncbi.nlm.nih.gov/pubmed/28149056
http://dx.doi.org/10.6026/97320630012202
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