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CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes

Carbon isotope fractionation (ε(p)) between the inorganic carbon source and organic matter has been proposed to be a function of pCO(2). To understand the CO(2)-dependency of ε(p) and species-specific differences therein, inorganic carbon fluxes in the four dinoflagellate species Alexandrium fundyen...

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Autores principales: Hoins, Mirja, Eberlein, Tim, Van de Waal, Dedmer B., Sluijs, Appy, Reichart, Gert-Jan, Rost, Björn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: North-Holland Pub. Co 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5268352/
https://www.ncbi.nlm.nih.gov/pubmed/28148970
http://dx.doi.org/10.1016/j.jembe.2016.04.001
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author Hoins, Mirja
Eberlein, Tim
Van de Waal, Dedmer B.
Sluijs, Appy
Reichart, Gert-Jan
Rost, Björn
author_facet Hoins, Mirja
Eberlein, Tim
Van de Waal, Dedmer B.
Sluijs, Appy
Reichart, Gert-Jan
Rost, Björn
author_sort Hoins, Mirja
collection PubMed
description Carbon isotope fractionation (ε(p)) between the inorganic carbon source and organic matter has been proposed to be a function of pCO(2). To understand the CO(2)-dependency of ε(p) and species-specific differences therein, inorganic carbon fluxes in the four dinoflagellate species Alexandrium fundyense, Scrippsiella trochoidea, Gonyaulax spinifera and Protoceratium reticulatum have been measured by means of membrane-inlet mass spectrometry. In-vivo assays were carried out at different CO(2) concentrations, representing a range of pCO(2) from 180 to 1200 μatm. The relative bicarbonate contribution (i.e. the ratio of bicarbonate uptake to total inorganic carbon uptake) and leakage (i.e. the ratio of CO(2) efflux to total inorganic carbon uptake) varied from 0.2 to 0.5 and 0.4 to 0.7, respectively, and differed significantly between species. These ratios were fed into a single-compartment model, and ε(p) values were calculated and compared to carbon isotope fractionation measured under the same conditions. For all investigated species, modeled and measured ε(p) values were comparable (A. fundyense, S. trochoidea, P. reticulatum) and/or showed similar trends with pCO(2) (A. fundyense, G. spinifera, P. reticulatum). Offsets are attributed to biases in inorganic flux measurements, an overestimated fractionation factor for the CO(2)-fixing enzyme RubisCO, or the fact that intracellular inorganic carbon fluxes were not taken into account in the model. This study demonstrates that CO(2)-dependency in ε(p) can largely be explained by the inorganic carbon fluxes of the individual dinoflagellates.
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spelling pubmed-52683522017-01-30 CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes Hoins, Mirja Eberlein, Tim Van de Waal, Dedmer B. Sluijs, Appy Reichart, Gert-Jan Rost, Björn J Exp Mar Bio Ecol Article Carbon isotope fractionation (ε(p)) between the inorganic carbon source and organic matter has been proposed to be a function of pCO(2). To understand the CO(2)-dependency of ε(p) and species-specific differences therein, inorganic carbon fluxes in the four dinoflagellate species Alexandrium fundyense, Scrippsiella trochoidea, Gonyaulax spinifera and Protoceratium reticulatum have been measured by means of membrane-inlet mass spectrometry. In-vivo assays were carried out at different CO(2) concentrations, representing a range of pCO(2) from 180 to 1200 μatm. The relative bicarbonate contribution (i.e. the ratio of bicarbonate uptake to total inorganic carbon uptake) and leakage (i.e. the ratio of CO(2) efflux to total inorganic carbon uptake) varied from 0.2 to 0.5 and 0.4 to 0.7, respectively, and differed significantly between species. These ratios were fed into a single-compartment model, and ε(p) values were calculated and compared to carbon isotope fractionation measured under the same conditions. For all investigated species, modeled and measured ε(p) values were comparable (A. fundyense, S. trochoidea, P. reticulatum) and/or showed similar trends with pCO(2) (A. fundyense, G. spinifera, P. reticulatum). Offsets are attributed to biases in inorganic flux measurements, an overestimated fractionation factor for the CO(2)-fixing enzyme RubisCO, or the fact that intracellular inorganic carbon fluxes were not taken into account in the model. This study demonstrates that CO(2)-dependency in ε(p) can largely be explained by the inorganic carbon fluxes of the individual dinoflagellates. North-Holland Pub. Co 2016-08 /pmc/articles/PMC5268352/ /pubmed/28148970 http://dx.doi.org/10.1016/j.jembe.2016.04.001 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hoins, Mirja
Eberlein, Tim
Van de Waal, Dedmer B.
Sluijs, Appy
Reichart, Gert-Jan
Rost, Björn
CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes
title CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes
title_full CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes
title_fullStr CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes
title_full_unstemmed CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes
title_short CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes
title_sort co(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5268352/
https://www.ncbi.nlm.nih.gov/pubmed/28148970
http://dx.doi.org/10.1016/j.jembe.2016.04.001
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