Cargando…
CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes
Carbon isotope fractionation (ε(p)) between the inorganic carbon source and organic matter has been proposed to be a function of pCO(2). To understand the CO(2)-dependency of ε(p) and species-specific differences therein, inorganic carbon fluxes in the four dinoflagellate species Alexandrium fundyen...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
North-Holland Pub. Co
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5268352/ https://www.ncbi.nlm.nih.gov/pubmed/28148970 http://dx.doi.org/10.1016/j.jembe.2016.04.001 |
_version_ | 1782500796699508736 |
---|---|
author | Hoins, Mirja Eberlein, Tim Van de Waal, Dedmer B. Sluijs, Appy Reichart, Gert-Jan Rost, Björn |
author_facet | Hoins, Mirja Eberlein, Tim Van de Waal, Dedmer B. Sluijs, Appy Reichart, Gert-Jan Rost, Björn |
author_sort | Hoins, Mirja |
collection | PubMed |
description | Carbon isotope fractionation (ε(p)) between the inorganic carbon source and organic matter has been proposed to be a function of pCO(2). To understand the CO(2)-dependency of ε(p) and species-specific differences therein, inorganic carbon fluxes in the four dinoflagellate species Alexandrium fundyense, Scrippsiella trochoidea, Gonyaulax spinifera and Protoceratium reticulatum have been measured by means of membrane-inlet mass spectrometry. In-vivo assays were carried out at different CO(2) concentrations, representing a range of pCO(2) from 180 to 1200 μatm. The relative bicarbonate contribution (i.e. the ratio of bicarbonate uptake to total inorganic carbon uptake) and leakage (i.e. the ratio of CO(2) efflux to total inorganic carbon uptake) varied from 0.2 to 0.5 and 0.4 to 0.7, respectively, and differed significantly between species. These ratios were fed into a single-compartment model, and ε(p) values were calculated and compared to carbon isotope fractionation measured under the same conditions. For all investigated species, modeled and measured ε(p) values were comparable (A. fundyense, S. trochoidea, P. reticulatum) and/or showed similar trends with pCO(2) (A. fundyense, G. spinifera, P. reticulatum). Offsets are attributed to biases in inorganic flux measurements, an overestimated fractionation factor for the CO(2)-fixing enzyme RubisCO, or the fact that intracellular inorganic carbon fluxes were not taken into account in the model. This study demonstrates that CO(2)-dependency in ε(p) can largely be explained by the inorganic carbon fluxes of the individual dinoflagellates. |
format | Online Article Text |
id | pubmed-5268352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | North-Holland Pub. Co |
record_format | MEDLINE/PubMed |
spelling | pubmed-52683522017-01-30 CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes Hoins, Mirja Eberlein, Tim Van de Waal, Dedmer B. Sluijs, Appy Reichart, Gert-Jan Rost, Björn J Exp Mar Bio Ecol Article Carbon isotope fractionation (ε(p)) between the inorganic carbon source and organic matter has been proposed to be a function of pCO(2). To understand the CO(2)-dependency of ε(p) and species-specific differences therein, inorganic carbon fluxes in the four dinoflagellate species Alexandrium fundyense, Scrippsiella trochoidea, Gonyaulax spinifera and Protoceratium reticulatum have been measured by means of membrane-inlet mass spectrometry. In-vivo assays were carried out at different CO(2) concentrations, representing a range of pCO(2) from 180 to 1200 μatm. The relative bicarbonate contribution (i.e. the ratio of bicarbonate uptake to total inorganic carbon uptake) and leakage (i.e. the ratio of CO(2) efflux to total inorganic carbon uptake) varied from 0.2 to 0.5 and 0.4 to 0.7, respectively, and differed significantly between species. These ratios were fed into a single-compartment model, and ε(p) values were calculated and compared to carbon isotope fractionation measured under the same conditions. For all investigated species, modeled and measured ε(p) values were comparable (A. fundyense, S. trochoidea, P. reticulatum) and/or showed similar trends with pCO(2) (A. fundyense, G. spinifera, P. reticulatum). Offsets are attributed to biases in inorganic flux measurements, an overestimated fractionation factor for the CO(2)-fixing enzyme RubisCO, or the fact that intracellular inorganic carbon fluxes were not taken into account in the model. This study demonstrates that CO(2)-dependency in ε(p) can largely be explained by the inorganic carbon fluxes of the individual dinoflagellates. North-Holland Pub. Co 2016-08 /pmc/articles/PMC5268352/ /pubmed/28148970 http://dx.doi.org/10.1016/j.jembe.2016.04.001 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hoins, Mirja Eberlein, Tim Van de Waal, Dedmer B. Sluijs, Appy Reichart, Gert-Jan Rost, Björn CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes |
title | CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes |
title_full | CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes |
title_fullStr | CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes |
title_full_unstemmed | CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes |
title_short | CO(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes |
title_sort | co(2)-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5268352/ https://www.ncbi.nlm.nih.gov/pubmed/28148970 http://dx.doi.org/10.1016/j.jembe.2016.04.001 |
work_keys_str_mv | AT hoinsmirja co2dependentcarbonisotopefractionationindinoflagellatesrelatestotheirinorganiccarbonfluxes AT eberleintim co2dependentcarbonisotopefractionationindinoflagellatesrelatestotheirinorganiccarbonfluxes AT vandewaaldedmerb co2dependentcarbonisotopefractionationindinoflagellatesrelatestotheirinorganiccarbonfluxes AT sluijsappy co2dependentcarbonisotopefractionationindinoflagellatesrelatestotheirinorganiccarbonfluxes AT reichartgertjan co2dependentcarbonisotopefractionationindinoflagellatesrelatestotheirinorganiccarbonfluxes AT rostbjorn co2dependentcarbonisotopefractionationindinoflagellatesrelatestotheirinorganiccarbonfluxes |