Cargando…

Deregulated lncRNAs in B Cells from Patients with Active Tuberculosis

Role of lncRNAs in human adaptive immune response to TB infection is largely unexplored. To address this issue, here we characterized lncRNA expression profile in primary human B cell response to TB infection using microarray assay. Several lncRNAs and mRNAs were chosen for RT-qPCR validation. Bioin...

Descripción completa

Detalles Bibliográficos
Autores principales: Fu, Yurong, Xu, Xianqin, Xue, Junfang, Duan, Wenping, Yi, Zhengjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5268381/
https://www.ncbi.nlm.nih.gov/pubmed/28125665
http://dx.doi.org/10.1371/journal.pone.0170712
_version_ 1782500803092676608
author Fu, Yurong
Xu, Xianqin
Xue, Junfang
Duan, Wenping
Yi, Zhengjun
author_facet Fu, Yurong
Xu, Xianqin
Xue, Junfang
Duan, Wenping
Yi, Zhengjun
author_sort Fu, Yurong
collection PubMed
description Role of lncRNAs in human adaptive immune response to TB infection is largely unexplored. To address this issue, here we characterized lncRNA expression profile in primary human B cell response to TB infection using microarray assay. Several lncRNAs and mRNAs were chosen for RT-qPCR validation. Bioinformatics prediction was applied to delineate function of the deregulated mRNAs. We found that 844 lncRNAs and 597 mRNAs were differentially expressed between B cell samples from individuals with or without TB. KEGG pathway analysis for the deregulated mRNAs indicated a number of pathways, such as TB, TLR signaling pathway and antigen processing and presentation. Moreover, corresponding to the dysregulation of many lncRNAs, we also found that their adjacent protein-coding genes were also deregulated. Functional annotation for the corresponding mRNAs showed that these lncRNAs were mainly associated with TLR signaling, TGF-β signaling. Interestingly, SOCS3, which is a critical negative regulator of cytokine response to TB infection and its nearby lncRNA XLOC_012582, were highly expressed in active TB B cells. Subsequent RT-qPCR results confirmed the changes. Whether upregulated XLOC_012582 causes SOCS3 overexpression and is eventually involved in the context of exacerbations of active TB represents an interesting issue that deserves to be further explored. Taken together, for the first time, we identified a set of deregulated lncRNAs in active TB B cells and their functions were predicted. Such findings provided novel insight into the pathogenesis of TB and further studies should focus on the function and pathogenic mechanisms of the lncRNAs involved in active TB.
format Online
Article
Text
id pubmed-5268381
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-52683812017-02-06 Deregulated lncRNAs in B Cells from Patients with Active Tuberculosis Fu, Yurong Xu, Xianqin Xue, Junfang Duan, Wenping Yi, Zhengjun PLoS One Research Article Role of lncRNAs in human adaptive immune response to TB infection is largely unexplored. To address this issue, here we characterized lncRNA expression profile in primary human B cell response to TB infection using microarray assay. Several lncRNAs and mRNAs were chosen for RT-qPCR validation. Bioinformatics prediction was applied to delineate function of the deregulated mRNAs. We found that 844 lncRNAs and 597 mRNAs were differentially expressed between B cell samples from individuals with or without TB. KEGG pathway analysis for the deregulated mRNAs indicated a number of pathways, such as TB, TLR signaling pathway and antigen processing and presentation. Moreover, corresponding to the dysregulation of many lncRNAs, we also found that their adjacent protein-coding genes were also deregulated. Functional annotation for the corresponding mRNAs showed that these lncRNAs were mainly associated with TLR signaling, TGF-β signaling. Interestingly, SOCS3, which is a critical negative regulator of cytokine response to TB infection and its nearby lncRNA XLOC_012582, were highly expressed in active TB B cells. Subsequent RT-qPCR results confirmed the changes. Whether upregulated XLOC_012582 causes SOCS3 overexpression and is eventually involved in the context of exacerbations of active TB represents an interesting issue that deserves to be further explored. Taken together, for the first time, we identified a set of deregulated lncRNAs in active TB B cells and their functions were predicted. Such findings provided novel insight into the pathogenesis of TB and further studies should focus on the function and pathogenic mechanisms of the lncRNAs involved in active TB. Public Library of Science 2017-01-26 /pmc/articles/PMC5268381/ /pubmed/28125665 http://dx.doi.org/10.1371/journal.pone.0170712 Text en © 2017 Fu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fu, Yurong
Xu, Xianqin
Xue, Junfang
Duan, Wenping
Yi, Zhengjun
Deregulated lncRNAs in B Cells from Patients with Active Tuberculosis
title Deregulated lncRNAs in B Cells from Patients with Active Tuberculosis
title_full Deregulated lncRNAs in B Cells from Patients with Active Tuberculosis
title_fullStr Deregulated lncRNAs in B Cells from Patients with Active Tuberculosis
title_full_unstemmed Deregulated lncRNAs in B Cells from Patients with Active Tuberculosis
title_short Deregulated lncRNAs in B Cells from Patients with Active Tuberculosis
title_sort deregulated lncrnas in b cells from patients with active tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5268381/
https://www.ncbi.nlm.nih.gov/pubmed/28125665
http://dx.doi.org/10.1371/journal.pone.0170712
work_keys_str_mv AT fuyurong deregulatedlncrnasinbcellsfrompatientswithactivetuberculosis
AT xuxianqin deregulatedlncrnasinbcellsfrompatientswithactivetuberculosis
AT xuejunfang deregulatedlncrnasinbcellsfrompatientswithactivetuberculosis
AT duanwenping deregulatedlncrnasinbcellsfrompatientswithactivetuberculosis
AT yizhengjun deregulatedlncrnasinbcellsfrompatientswithactivetuberculosis