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A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area

Fungal growth inhibition on solid media has been historically measured and calculated based on the average of perpendicular diameter measurements of growth on fungicide amended media. We investigated the sensitivity of the calculated area (D(A)) and the measured area (M(A)) for assessing fungicide g...

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Detalles Bibliográficos
Autores principales: Hendricks, Katherine E., Christman, Mary C., Roberts, Pamela D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5268463/
https://www.ncbi.nlm.nih.gov/pubmed/28125679
http://dx.doi.org/10.1371/journal.pone.0170755
Descripción
Sumario:Fungal growth inhibition on solid media has been historically measured and calculated based on the average of perpendicular diameter measurements of growth on fungicide amended media. We investigated the sensitivity of the calculated area (D(A)) and the measured area (M(A)) for assessing fungicide growth inhibition of the ascomycete, Phyllosticta citricarpa on solid media. Both the calculated, D(A) and the actual measured area, M(A) were adequate for distinguishing significant treatment effects of fungicide on fungal growth, however M(A) was more sensitive at identifying significant differences between the controls and fungicide concentrations below 5 ppm.