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Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo

Osteoporosis, which is a systemic skeletal disease characterized by low bone mineral density and microarchitectural deterioration of bone quality, is a global and increasing public health problem. Recent studies have suggested that Tenuigenin (TEN), a class of native compounds with numerous biologic...

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Autores principales: Jiang, Hua-ji, Tian, Xing-gui, Huang, Shou-bin, Chen, Guo-rong, Huang, Min-jun, Chen, Yu-hui, Yan, Bin, Li, Sheng-fa, Tang, Jia-jun, Zhao, Hui-yu, Wang, Liang, Zhang, Zhong-min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5269466/
https://www.ncbi.nlm.nih.gov/pubmed/27844205
http://dx.doi.org/10.1007/s00441-016-2528-1
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author Jiang, Hua-ji
Tian, Xing-gui
Huang, Shou-bin
Chen, Guo-rong
Huang, Min-jun
Chen, Yu-hui
Yan, Bin
Li, Sheng-fa
Tang, Jia-jun
Zhao, Hui-yu
Wang, Liang
Zhang, Zhong-min
author_facet Jiang, Hua-ji
Tian, Xing-gui
Huang, Shou-bin
Chen, Guo-rong
Huang, Min-jun
Chen, Yu-hui
Yan, Bin
Li, Sheng-fa
Tang, Jia-jun
Zhao, Hui-yu
Wang, Liang
Zhang, Zhong-min
author_sort Jiang, Hua-ji
collection PubMed
description Osteoporosis, which is a systemic skeletal disease characterized by low bone mineral density and microarchitectural deterioration of bone quality, is a global and increasing public health problem. Recent studies have suggested that Tenuigenin (TEN), a class of native compounds with numerous biological activities such as anti-resorptive properties, exerts protective effects against postmenopausal bone loss. The present study aims to investigate the osteogenic effects of TEN on bone mesenchymal stem cells (BMSCs) in vitro and in vivo. Alkaline phosphatase (ALP) activity/staining, Alizarin red staining and the expression of osteogenic markers, including runt-related transcription factor 2, osterix, osteocalcin, collagen Iα1, β-catenin and glycogen synthase kinase-3β were investigated in primary femoral BMSCs from C57/BL6 mice cultured under osteogenic conditions for 2 weeks to examine the effects of TEN. An ovariectomized (OVX) mouse model was used to investigate the effect of TEN treatment for 3 months in vivo. We found that ALP activity, mineralized nodules and the expression of osteogenic markers were increased and WNT/β-catenin signaling was enhanced in vitro and in vivo. Bone parameters, including trabecular thickness, trabecular number and bone mineral density were higher in the OVX+TEN group than in control OVX mice. Our results suggest the therapeutic potential of TEN for the treatment of patients with postmenopausal osteoporosis.
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spelling pubmed-52694662017-02-09 Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo Jiang, Hua-ji Tian, Xing-gui Huang, Shou-bin Chen, Guo-rong Huang, Min-jun Chen, Yu-hui Yan, Bin Li, Sheng-fa Tang, Jia-jun Zhao, Hui-yu Wang, Liang Zhang, Zhong-min Cell Tissue Res Regular Article Osteoporosis, which is a systemic skeletal disease characterized by low bone mineral density and microarchitectural deterioration of bone quality, is a global and increasing public health problem. Recent studies have suggested that Tenuigenin (TEN), a class of native compounds with numerous biological activities such as anti-resorptive properties, exerts protective effects against postmenopausal bone loss. The present study aims to investigate the osteogenic effects of TEN on bone mesenchymal stem cells (BMSCs) in vitro and in vivo. Alkaline phosphatase (ALP) activity/staining, Alizarin red staining and the expression of osteogenic markers, including runt-related transcription factor 2, osterix, osteocalcin, collagen Iα1, β-catenin and glycogen synthase kinase-3β were investigated in primary femoral BMSCs from C57/BL6 mice cultured under osteogenic conditions for 2 weeks to examine the effects of TEN. An ovariectomized (OVX) mouse model was used to investigate the effect of TEN treatment for 3 months in vivo. We found that ALP activity, mineralized nodules and the expression of osteogenic markers were increased and WNT/β-catenin signaling was enhanced in vitro and in vivo. Bone parameters, including trabecular thickness, trabecular number and bone mineral density were higher in the OVX+TEN group than in control OVX mice. Our results suggest the therapeutic potential of TEN for the treatment of patients with postmenopausal osteoporosis. Springer Berlin Heidelberg 2016-11-14 2017 /pmc/articles/PMC5269466/ /pubmed/27844205 http://dx.doi.org/10.1007/s00441-016-2528-1 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Regular Article
Jiang, Hua-ji
Tian, Xing-gui
Huang, Shou-bin
Chen, Guo-rong
Huang, Min-jun
Chen, Yu-hui
Yan, Bin
Li, Sheng-fa
Tang, Jia-jun
Zhao, Hui-yu
Wang, Liang
Zhang, Zhong-min
Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo
title Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo
title_full Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo
title_fullStr Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo
title_full_unstemmed Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo
title_short Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo
title_sort tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5269466/
https://www.ncbi.nlm.nih.gov/pubmed/27844205
http://dx.doi.org/10.1007/s00441-016-2528-1
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