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ComB proteins expression levels determine Helicobacter pylori competence capacity

Helicobacter pylori chronically colonises half of the world’s human population and is the main cause of ulcers and gastric cancers. Its prevalence and the increase in antibiotic resistance observed recently reflect the high genetic adaptability of this pathogen. Together with high mutation rates and...

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Autores principales: Corbinais, Christopher, Mathieu, Aurélie, Damke, Prashant P., Kortulewski, Thierry, Busso, Didier, Prado-Acosta, Mariano, Radicella, J. Pablo, Marsin, Stéphanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5269756/
https://www.ncbi.nlm.nih.gov/pubmed/28128333
http://dx.doi.org/10.1038/srep41495
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author Corbinais, Christopher
Mathieu, Aurélie
Damke, Prashant P.
Kortulewski, Thierry
Busso, Didier
Prado-Acosta, Mariano
Radicella, J. Pablo
Marsin, Stéphanie
author_facet Corbinais, Christopher
Mathieu, Aurélie
Damke, Prashant P.
Kortulewski, Thierry
Busso, Didier
Prado-Acosta, Mariano
Radicella, J. Pablo
Marsin, Stéphanie
author_sort Corbinais, Christopher
collection PubMed
description Helicobacter pylori chronically colonises half of the world’s human population and is the main cause of ulcers and gastric cancers. Its prevalence and the increase in antibiotic resistance observed recently reflect the high genetic adaptability of this pathogen. Together with high mutation rates and an efficient DNA recombination system, horizontal gene transfer through natural competence makes of H. pylori one of the most genetically diverse bacteria. We show here that transformation capacity is enhanced in strains defective for recN, extending previous work with other homologous recombination genes. However, inactivation of either mutY or polA has no effect on DNA transformation, suggesting that natural competence can be boosted in H. pylori by the persistence of DNA breaks but not by enhanced mutagenesis. The transformation efficiency of the different DNA repair impaired strains correlates with the number of transforming DNA foci formed on the cell surface and with the expression of comB8 and comB10 competence genes. Overexpression of the comB6-B10 operon is sufficient to increase the transformation capacity of a wild type strain, indicating that the ComB complex, present in the bacterial wall and essential for DNA uptake, can be a limiting factor for transformation efficiency.
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spelling pubmed-52697562017-02-01 ComB proteins expression levels determine Helicobacter pylori competence capacity Corbinais, Christopher Mathieu, Aurélie Damke, Prashant P. Kortulewski, Thierry Busso, Didier Prado-Acosta, Mariano Radicella, J. Pablo Marsin, Stéphanie Sci Rep Article Helicobacter pylori chronically colonises half of the world’s human population and is the main cause of ulcers and gastric cancers. Its prevalence and the increase in antibiotic resistance observed recently reflect the high genetic adaptability of this pathogen. Together with high mutation rates and an efficient DNA recombination system, horizontal gene transfer through natural competence makes of H. pylori one of the most genetically diverse bacteria. We show here that transformation capacity is enhanced in strains defective for recN, extending previous work with other homologous recombination genes. However, inactivation of either mutY or polA has no effect on DNA transformation, suggesting that natural competence can be boosted in H. pylori by the persistence of DNA breaks but not by enhanced mutagenesis. The transformation efficiency of the different DNA repair impaired strains correlates with the number of transforming DNA foci formed on the cell surface and with the expression of comB8 and comB10 competence genes. Overexpression of the comB6-B10 operon is sufficient to increase the transformation capacity of a wild type strain, indicating that the ComB complex, present in the bacterial wall and essential for DNA uptake, can be a limiting factor for transformation efficiency. Nature Publishing Group 2017-01-27 /pmc/articles/PMC5269756/ /pubmed/28128333 http://dx.doi.org/10.1038/srep41495 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Corbinais, Christopher
Mathieu, Aurélie
Damke, Prashant P.
Kortulewski, Thierry
Busso, Didier
Prado-Acosta, Mariano
Radicella, J. Pablo
Marsin, Stéphanie
ComB proteins expression levels determine Helicobacter pylori competence capacity
title ComB proteins expression levels determine Helicobacter pylori competence capacity
title_full ComB proteins expression levels determine Helicobacter pylori competence capacity
title_fullStr ComB proteins expression levels determine Helicobacter pylori competence capacity
title_full_unstemmed ComB proteins expression levels determine Helicobacter pylori competence capacity
title_short ComB proteins expression levels determine Helicobacter pylori competence capacity
title_sort comb proteins expression levels determine helicobacter pylori competence capacity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5269756/
https://www.ncbi.nlm.nih.gov/pubmed/28128333
http://dx.doi.org/10.1038/srep41495
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