Improved results of LINE-1 methylation analysis in formalin-fixed, paraffin-embedded tissues with the application of a heating step during the DNA extraction process

BACKGROUND: Formalin-fixed, paraffin-embedded (FFPE) tissues are important resources for profiling DNA methylation changes and for studying a variety of diseases. However, formalin fixation introduces inter-strand crosslinking, which might cause incomplete bisulfite conversion of unmethylated cytosi...

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Detalles Bibliográficos
Autores principales: Wen, Xianyu, Jeong, Seorin, Kim, Younghoon, Bae, Jeong Mo, Cho, Nam Yun, Kim, Jung Ho, Kang, Gyeong Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5270344/
https://www.ncbi.nlm.nih.gov/pubmed/28149329
http://dx.doi.org/10.1186/s13148-016-0308-0

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