Cargando…
Proteomic profiling of mitochondria: what does it tell us about the ageing brain?
Mitochondrial dysfunction is evident in numerous neurodegenerative and age-related disorders. It has also been linked to cellular ageing, however our current understanding of the mitochondrial changes that occur are unclear. Functional studies have made some progress reporting reduced respiration, d...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5270661/ https://www.ncbi.nlm.nih.gov/pubmed/27992860 http://dx.doi.org/10.18632/aging.101131 |
_version_ | 1782501206681190400 |
---|---|
author | Ingram, Thomas Chakrabarti, Lisa |
author_facet | Ingram, Thomas Chakrabarti, Lisa |
author_sort | Ingram, Thomas |
collection | PubMed |
description | Mitochondrial dysfunction is evident in numerous neurodegenerative and age-related disorders. It has also been linked to cellular ageing, however our current understanding of the mitochondrial changes that occur are unclear. Functional studies have made some progress reporting reduced respiration, dynamic structural modifications and loss of membrane potential, though there are conflicts within these findings. Proteomic analyses, together with functional studies, are required in order to profile the mitochondrial changes that occur with age and can contribute to unravelling the complexity of the ageing phenotype. The emergence of improved protein separation techniques, combined with mass spectrometry analyses has allowed the identification of age and cell-type specific mitochondrial changes in energy metabolism, antioxidants, fusion and fission machinery, chaperones, membrane proteins and biosynthesis pathways. Here, we identify and review recent data from the analyses of mitochondria from rodent brains. It is expected that knowledge gained from understanding age-related mitochondrial changes of the brain should lead to improved biomarkers of normal ageing and also age-related disease progression. |
format | Online Article Text |
id | pubmed-5270661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-52706612017-01-27 Proteomic profiling of mitochondria: what does it tell us about the ageing brain? Ingram, Thomas Chakrabarti, Lisa Aging (Albany NY) Review Mitochondrial dysfunction is evident in numerous neurodegenerative and age-related disorders. It has also been linked to cellular ageing, however our current understanding of the mitochondrial changes that occur are unclear. Functional studies have made some progress reporting reduced respiration, dynamic structural modifications and loss of membrane potential, though there are conflicts within these findings. Proteomic analyses, together with functional studies, are required in order to profile the mitochondrial changes that occur with age and can contribute to unravelling the complexity of the ageing phenotype. The emergence of improved protein separation techniques, combined with mass spectrometry analyses has allowed the identification of age and cell-type specific mitochondrial changes in energy metabolism, antioxidants, fusion and fission machinery, chaperones, membrane proteins and biosynthesis pathways. Here, we identify and review recent data from the analyses of mitochondria from rodent brains. It is expected that knowledge gained from understanding age-related mitochondrial changes of the brain should lead to improved biomarkers of normal ageing and also age-related disease progression. Impact Journals LLC 2016-12-15 /pmc/articles/PMC5270661/ /pubmed/27992860 http://dx.doi.org/10.18632/aging.101131 Text en Copyright: © 2016 Ingram and Chakrabarti http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Review Ingram, Thomas Chakrabarti, Lisa Proteomic profiling of mitochondria: what does it tell us about the ageing brain? |
title | Proteomic profiling of mitochondria: what does it tell us about the ageing brain? |
title_full | Proteomic profiling of mitochondria: what does it tell us about the ageing brain? |
title_fullStr | Proteomic profiling of mitochondria: what does it tell us about the ageing brain? |
title_full_unstemmed | Proteomic profiling of mitochondria: what does it tell us about the ageing brain? |
title_short | Proteomic profiling of mitochondria: what does it tell us about the ageing brain? |
title_sort | proteomic profiling of mitochondria: what does it tell us about the ageing brain? |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5270661/ https://www.ncbi.nlm.nih.gov/pubmed/27992860 http://dx.doi.org/10.18632/aging.101131 |
work_keys_str_mv | AT ingramthomas proteomicprofilingofmitochondriawhatdoesittellusabouttheageingbrain AT chakrabartilisa proteomicprofilingofmitochondriawhatdoesittellusabouttheageingbrain |